Anti-MSN Antibody, clone 2C12

32160702

NA.41

Moesin (membrane-organizing extension spike protein) is the protein encoded by the MSN gene. The MSN encoded protein moesin is a member of the ERM (ezrin, moesin, radixin) family of proteins that regulate linkage between the plasma membrane and the actin cytoskeleton. Thus, the MSN encoded protein is a peripheral membrane protein that is keenly involved in the signaling and structural connections in cytoskeletal plasma membrane structures like microvilli. Moesin in its active, phosphorylated state strongly binds actin and helps anchor it to the plasma membrane. The MSN encoded Moesin is localized to filopodia and other membranous extensions of various cell types and thus plays a role in cell-cell recognition signaling, cell movement, and cell transport. In its inactive, non-phosphorylated state, Moesin binds itself in a head-tail fashion and is incapable of binding actin or other cytoskeletal proteins. The kinase ROCK2 is central to the opening up of moesin and allowing its interaction with actin and the plasma membrane. EMD-Millipore’s Anti-MSN, clone 2C12 has been tested in western blots against purified recombinant protein as well as multiple human cancer cell lysates. The clone has also been tested successfully in paraffin embedded immunohistochemistry on human colon tissue and by flow cytometry on human Jurkat cells and ELISA using purified recombinant antigen.

Purified protein fragment of human MSN expressed in E. coli.

Detect Moesin using this mouse monoclonal antibody, Anti-MSN Antibody, clone 2C12 validated for use in western blotting, IHC & Flow Cytometry.
Immunohistochemistry Analysis: A 1:100-1,000 dilution from a representative lot detected MSN in human tonsil and colon tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected MSN in Jurkat cells.

Optimal working dilutions must be determined by end user.

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected MSN in 10 µg of Jurkat cell lysate.

100

mouse

ascites fluid

primary antibodies

2C12, monoclonal

human

flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

IgG1

NP_002435

P26038

wet ice

human ... MSN(4478)

~68 kDa observed. Uncharacterized bands may appear in some lysate(s).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

GHS08

Danger

H360D - H412

P201 - P202 - P273 - P280 - P308 + P313 - P405

Aquatic Chronic 3 - Repr. 1A

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK 2