Anti-monomethyl-Histone H3 Antibody (Lys36)

32160702

NA.41

Histone H3 is one of four histones that form a core octameric structure that is required for the packaging of DNA into nucleosomes, the most basic level of chromatin arrangement. Histones undergo a number of post-translational modifications (PTM) at their N- and C-terminal tails which can alter chromatin structure and stability, and influence DNA repair and gene expression. Histone H3 undergoes methylation of its lysine and arginine residues, with varying biological outcomes. The lysine 36 residue undergoes mono-, di-, or trimethylation which may be performed by one of many methyltransferase enzymes (SET2, NSD1, NSD2, NSD3, ASH1, SMYD2, SETMAR, KMT3A, and SETD3). Several studies have indicated that methylated lysine 36 residues promote Rpd3S-mediated deacetylation of other histones resulting in gene activation.

This antibody recognizes the N-terminus of Histone H3 monomethylated at Lys36.

Epitope: Monomethylated Lys36
KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H3 monomethylated at Lys36.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated monomethyl-Histone H3 (Lys36) from HeLa acid extract.

Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see table) were probed with Anti-monomethyl-Histone H3 (Lys36) (0.5 µg/mL).
Anti-monomethyl-Histone H3 (Lys36) Antibody is a rabbit polyclonal antibody for detection of Histone H3 monomethylated at lysine 36. This purified polyclonal antibody also known as Anti-H3K36me1, has dot blot (DB) proven specificity & has been validated in WB, IP.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function

Evaluated by Western Blot in HeLa acid extract and various recombinant Histone proteins.

Western Blot Analysis: 0.05 µg/mL of this antibody detected monomethyl-Histone H3 (Lys36) in 10 µg of HeLa acid extract.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Control
HeLa acid extract and various recombinant Histone proteins

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

rat, mouse, human

dot blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NP_003484

Q16695

wet ice

human ... HIST1H3F(8968)

~17 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

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