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Anti-monomethyl Histone H3 Antibody (Lys18)
| 产品编号： | 4110346 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-monomethyl Histone H3 Antibody (Lys18)
Yeast (S.cerevisiae) partial histone 3 (H3) gene, H3K18me1
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. |
| Immunogen【免疫原】 | Linear peptide corresponding to yeast Histone H3 monomethylated at Lys18. |
| Application【应用】 | Research Sub Category Histones Detect methylated Histone H3 using this rabbit polyclonal antibody, Anti-monomethyl Histone H3 Antibody (Lys18) validated for use in western blotting & Dot Blot. Research Category Epigenetics & Nuclear Function Western Blotting Analysis: A representative lot detected monomethyl Histone H3 (Lys18) in yeast whole cell extracts, which express wild-type monomethyl Histone H3 (Lys18) and not mutant monomethyl Histone H3 (Lys18Ala) (Prof. F. Howe from the laboratory of Prof. J. Mellor, University of Oxford.). Dot Blot Analysis: A representative lot detected monomethyl Histone H3 (Lys18) peptide and not unmodified Histone H3 (Prof. F. Howe from the laboratory of Prof. J. Mellor, University of Oxford.). Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated monomethyl Histone H3 (Lys18) in 1.6X 10E7 yeast cell equivalents (Prof. F. Howe from the laboratory of Prof. J. Mellor, University of Oxford.). |
| Quality【质量】 | Evaluated by Western Blotting in HeLa acid extract. Western Blotting Analysis: 1:1,000 dilution of this antibody detected monomethyl Histone H3 (Lys18) in 10 µg of HeLa acid extract. |
| Physical form【外形】 | Affinity purified Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Legal Information【法律信息】 | GenBank is a registered trademark of United States Department of Health and Human Services |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | yeast, human |
| technique(s) | ChIP: suitable dot blot: suitable western blot: suitable |
| GenBank® accession no. | AAA34659 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~17 kDa observed |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
