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Anti-LbCas12a (Cpf1) antibody, Mouse monoclonal
产品编号: | 4109692 |
规格: | clone LbCpf1, purified from hybridoma cell culture |
包装规格: | 100 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-LbCas12a (Cpf1) antibody, Mouse monoclonal
Anti-CRISPR-associated endonuclease AsCas12a from Lachnospiraceae bacterium ND2006
Anti-LbCpf1 antibody, Mouse monoclonal
基本信息
NACRES | NA.41 |
General description【一般描述】 | Clustered, regularly interspaced, short palindromic repeat (CRISPR) systems encode RNA-guided endonucleases that are essential for bacterial adaptive immunity. Depending on the architecture of the effector-CRISPR RNA (crRNA) interference module, different CRISPR-Cas systems could be assigned into two classes1: class 1 systems of multi-subunit complex, such as Cascade and class 2 systems of single enzyme, such as Cas9. Cpf1 (CRISPR from Prevotella and Francisella 1) belongs to class 2 type V CRISPR-Cas endonuclease system. Cpf1 comprise several differences from Cas9 protein including cleavage with 5′ overhangs, a shorter guide RNA and a longer distance between the seed sequence and cleavage site. LbCpf1, Cpf1 from Lachnospiraceae bacterium ND2006, was examined together with 15 members of Cpf1 nuclease family and proved to mediate efficient genome editing in HEK293FT cells with improved results compared to SpCas9.5 According to thr crystal structure, LbCpf1 has a triangle-shaped architecture with a large positively charged channel at the centre.7 The crRNA binding was shown to induce the pronounced structural rearrangements of LbCpf1, leading to formation of a substrate-binding conformation of LbCpf1. Over-expressed in plant cells, LbCpf1 demonstrated more than 10-fold transcriptional repression of the target gene. Monoclonal anti-LbCpf1 antibody can provide a useful tool for genome editing research, such as detecting and monitoring LbCpf1 positively transfected cells. |
Immunogen【免疫原】 | Recombinant LbCpf1 |
Application【应用】 | Monoclonal Anti-LbCpf1 specifically recognizes Cpf1 from Lachnospiraceae bacterium ND2006. The product may be used in several immunochemical techniques including Immunoblotting (∼135 kDa), Immunofluorescence and Immunoprecipitation. Monoclonal Anti-LbCpf1 does not cross react with Cpf1 from Acidaminococcus sp. (strain BV3L6) ), SpCas9 from Streptococcus pyogenes bacteria and FnCas9 from Francisella novicida bacteria. |
Physical form【外形】 | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Other Notes【其他说明】 | This product is for R&D use only, not for drug, household, or other uses. In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test. |
产品性质
Quality Level【质量水平】 | 200 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified from hybridoma cell culture |
antibody product type | primary antibodies |
clone【克隆】 | LbCpf1, monoclonal |
form【形式】 | buffered aqueous solution |
mol wt【分子量】 | ~135 kDa |
concentration【浓度】 | ~1.0 mg/mL |
technique(s) | immunoblotting: 0.6-1.2 μg/mL using human HEK-293T cells over-expressing LbCpf1 protein immunofluorescence: 0.25-0.5 μg/mL using human HEK-293T cells over-expressing LbCpf1 protein immunoprecipitation (IP): 1-2.5 μg/test using lysate of human HEK-293T cells over-expressing LbCpf1 protein |
isotype【同位素/亚型】 | IgG1 |
UniProt accession no.【UniProt登记号】 | A0A182DWE3 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
产品说明
Storage and Stability【储存及稳定性】 | For continuous use, store at 2-8 ℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | nwg |