Anti-Laminin-1 A&B chains Antibody, cross region, clone AL-2

32160702

NA.41

Laminins are secreted glycoproteins incorporated into cell-associated extracellular matrices and an integral part of the structural scaffolding in most animal tissues. The laminin protein is made up of three polypeptide chains; α, β and γ, accounting for its flexibility in connecting various molecules. When originally characterized, laminin-1 clone AL-2 reacted with Engelbreth-Holm-Swarm (EHS) mouse laminin A & B chains on the long arm near the cross region, (Skubitz, 1987). More recently, investigations, (Schèele, 2006) have shown that the clone binds laminin β1 under reducing conditions as well as native ELISAs and that the affinity is increased by the presence of the laminin α1 chain. Thus there is significant laminin β1 reactivity with clone AL-2.

Reacts with EHS mouse laminin A & B chains on the long arm near the cross region.
Other species have not been tested.

Epitope: Near cross region
Laminin isolated from EHS mouse sarcoma.

Research Category
Cell Structure
Detect Laminin-1 A&B chains using this Anti-Laminin-1 A&B chains Antibody, cross region, clone AL-2 validated for use in ELISA, IF, IH(P), IP & WB.
Immunofluorescence:
An independent laboratory (Schèele, S., 2006) has verified anti-laminin clone AL-2 to work using immunofluorescence techniques on mouse muscle tissue, including both transgenic and wild-type tissues.
Research Sub Category
ECM Proteins

Evaluated on a representative lot by Western blot on purified mouse laminin-1.

Format: Purified
Purified in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% NaN3.
Protein G Purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

rat

purified antibody

primary antibodies

AL-2, monoclonal

mouse

Chemicon^®

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

IgG1κ

NM_005559.2

P25391
P55268

wet ice

~200 kDa

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1