Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2

32160702

NA.41

Kinesins are molecular motors used for intracellular transport and trafficking. They move along microtubule cables powered by the hydrolysis of ATP, thus kinesins are ATPases. The active movement of kinesins supports several cellular functions including mitosis, meiosis and transport of cargo such as axonal transport.

Reacts with the heavy chain of kinesin near the N-terminus.

Epitope: a.a.420-445
Bovine brain kinesin.

Research Category
Cell Structure
Immunohistochemistry:
A previous lot was used on paraformaldehyde fixed tissue. Also works for EM.

Immunocytochemistry:
A previous lot of this antibody was used in Immunocytochemistry.

Immunoprecipitation:
A previous lot of this antibody was used in Immunoprecipitation.

EIA/RIA:
A previous lot of this antibody was used in RIA.

Immunoblotting: recognizes 124kDa KHC and its proteolytic fragments.

Inhibition of kinesin mediated transport {Brady, et al PNAS 87(3):1061-1065, 1990.

Optimal working dilutions must be determined by the end user.
Research Sub Category
Cytoskeleton
Detect Kinesin using this Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2 validated for use in ELISA, IC, IH, IP, FUNC, RIA & WB.

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected KINESIN (HEAVY CHAIN) on 10 μg of Mouse Brain lysates.

Format: Purified
Protein A purified
Purified mouse monoclonal IgG2b in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.

Control
Brain tissue

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified immunoglobulin

primary antibodies

H2, monoclonal

human, chicken, rat, sea urchin, squid, mouse

mammals

Chemicon^®

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable

IgG2b

NP_004512

P33176

wet ice

124 kDa

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2