Anti-Integrin beta1 (CD29) Antibody, clone mAb13

32160702

NA.41

Integrin beta-1 (UniProt: P05556; also known as Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12) gene (Gene ID: 3688) in human. Integrin beta-1 is a single-pass type I membrane protein that can associate with one of the multiple alpha subunits to serve as a receptor for fibronectin and other extracellular matrix proteins. Integrin beta-1 integrin recognizes the sequence R-G-D in a wide array of ligands. Five isoforms of integrin beta-1 have been reported that are produced by alternative splicing. Integrin beta-1 is synthesized with a signal peptide (aa 1-20) that is subsequently cleaved off. The mature form has an extracellular domain (aa 21-728), a helical domain (aa 729-751) and a cytoplasmic region (aa 752-798). Isoform 1 of integrin beta-1 is widely expressed and other isoforms are generally co-expressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4 is selectively expressed in peripheral T-cells and isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle). Isoform 2 is reported to interfere with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro).

Clone mAb13 specifically detects beta 1 subunit of human integrin. It targets an epitope within 12 amino acids from the N-terminal half.

Fibronectin receptor (alpha V beta 1) purified from human placenta.

Research Category
Cell Structure
Inhibition Analysis: A representative lot blocked cell attachment in Inhibition studies (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75; Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43).

Immunocytochemistry Analysis: A representative lot detected Integrin beta1 (CD29) in Immunocytochemistry applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75; Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43).

Immunoprecipitation Analysis: A representative lot detected Integrin beta1 (CD29) in Immunoprecipitation applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75).

Inhibition Analysis: A representative lot blocked HT-1080 cells adhesion to fibronectin coated plates (Courtesy of Dr. Kenneth M. Yamada, M.D., Ph.D., NIDCR, National Institutes of Health, Bethesda, MD USA).

Anti-Integrin beta1 (CD29), clone mAb13, Cat. No. MABT821, is a rat monoclonal antibody that detects integrin beta-1 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Inhibition studies.

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Integrin beta1 (CD29) in A431 cells.

Protein G purified
Purified rat monoclonal antibody IgG2a in PBS without preservatives.

Format: Purified

Concentration: Please refer to lot specific datasheet.

100

rat

purified immunoglobulin

primary antibodies

mAb13, monoclonal

human

antibody small pack of 25 μL

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable

IgG2aκ

NP_596867

P05556

ambient

human ... ITGB1(3688)

88.42 kDa calculated.

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.