Anti-Integrin α6 Antibody, clone BnC6B4

32160702

NA.41

Reactive with chicken integrin alpha6, as demonstrated by experiments with cDNA-transfected NIH3T3 cells followed by immunoblots.

Integrin alpha subunits purified from adult chicken brain tissue, denatured in 6M guanidine isothiocyanate, and depleted of beta1 subunit.

Anti-Integrin α6 Antibody, clone BnC6B4 is an antibody against Integrin α6 for use in IP, WB, IC, IH.
Western blot

Immunoprecipitation: Precipitates the monomeric alpha6 subunit after the dimeric receptor has been denatured, but does not precipitate the native receptor.

Immunohistochemistry: Effective for immunohistochemistry of methanol-fixed cryosections. An overnight methanol fix is suggested. Previous experiments have used 14 micron sections of chicken embryonic tissue. Typical antibodies dilutions are 35 μg/mL, with overnight antibody incubation, followed by detection with a goat anti-mouse Ig -FITC secondary antiobody.

Immunocytochemistry: Effective for methanol-fixed cultured cells (15 minute fixation).

Immunoblot: Recognizes a 130 kDa band in chicken brain tissue and a 140 kDa band in transfected NIH3T3 cells (the difference in molecular weight is likely due to differential glycosylation).

Optimal working dilutions must be determined by end user.

Format: Purified

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified immunoglobulin

primary antibodies

BnC6B4, monoclonal

chicken

Chemicon^®

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG1

NM_000210.2
NM_001079818.1

P23229

wet ice

human ... ITGA6(3655)

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Manufactured by Daiichi Fine Chemical Co., Ltd

10 - Combustible liquids

WGK 2