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Anti-Integrin α1 Antibody, clone FB12
产品别名
Anti-Integrin α1 Antibody, clone FB12
CD49a
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
Specificity【特异性】 | Reacts with the I domain (Val151 - Ala364) of human alpha-1 integrin (CD49a)1. Precipitates proteins of Mr 180 kDa, pI 5.9-6.0 (alpha chain) and Mr 115 kDa, pI 4.5-5.0 (beta chain) from human lymphocyte lysates (Zocchi et al., 1991). |
Application【应用】 | This Anti-Integrin α1 Antibody, clone FB12 is validated for use in ELISA, FC, IC, IH, IP, FUNC, WB for the detection of Integrin α1. Immunohistochemistry: 2 μg/mL, with paraformaldehyde fixation. Immunohistochemistry: Frozen sections (7 µm) of placental tissue fixed with acetone for 1 hr. Antibody used at 1:10 dilution (Sato et al., 2003). Immunocytochemistry: iolated EVT cells firxed with 0.5% PFA @ 4°C for 15 minutes followed by acetone at -20°C for 2 minutes. Antibody was used at 1:10 dilution (Sato et al., 2003). Immunoblotting: 2 μg/mL Immunoprecipitation: 1 μg/mL FACS analysis: 2μg/mL (Sato et al., 2005). ELISA: 0.5 μg/mL Inhibition of activated human lymphocyte binding to laminin, collagen IV and fibronectin (Fabbri et al., 1996): 1-5 μg/mL Optimal working dilutions must be determined by end user. |
Physical form【外形】 | Protein A purified Purified immunoglobulin in 0.02M PB, pH 7.6, 0.25M NaCl containing 0.1% sodium azide. Format: Purified |
Analysis Note【分析说明】 | Control Microvascular endothelium |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | FB12, monoclonal |
species reactivity | human |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG1 |
NCBI accession no.【NCBI登记号】 | NM_181501.1 |
UniProt accession no.【UniProt登记号】 | P56199 |
shipped in【运输】 | wet ice |
Gene Information | human ... ITGA1(3672) |
产品说明
Target description【目标描述】 | 130 kDa |
【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 2 |