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Anti-Human IgM (μ-chain specific) antibody, Mouse monoclonal
| 产品编号： | 4109014 |
| 规格： | clone MB-11, purified from hybridoma cell culture |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-Human IgM (μ-chain specific) antibody, Mouse monoclonal
Anti-Human immunoglobulin M
基本信息
| NACRES | NA.46 |
| General description【一般描述】 | Immunoglobulin M (IgM) is the major class of surface immunoglobulins on lymphocyte membranes. IgM is suggested to act as the first line of defense during microbial infections. IgM has a pentameric structure, in which monomers are linking together via disulfide bonds. Surface IgM is expressed on immature and mature B cells, whereas IgM heavy chain is expressed intracellularly in pre-B cells. IgM serves as the antigen receptor of naive B cells and is involved in B cell maturation and complement activation. Anti-Human IgM (m-chain specific) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the MB-11 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgM protein. |
| Specificity【特异性】 | Anti-Human IgM (m-chain specific) antibody, Mouse monoclonal specifically recognizes m chain of Human IgM. The antibody shows no cross-reactivity with human IgG, Fab, Fc, k, l, and g chains. |
| Immunogen【免疫原】 | Purified human IgM |
| Application【应用】 | The antibody is recommended to use in various immunological techniques, including ELISA and Immunoblot. |
| Biochem/physiol Actions【生化/生理作用】 | Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions. IgM serves as the antigen receptor of naive B cells and is involved in B cell maturation and complement activation. |
| Physical form【外形】 | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative. |
| Other Notes【其他说明】 | This product is for R&D use only, not for drug, household, or other uses. |
产品性质
| Quality Level【质量水平】 | 300 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone【克隆】 | MB-11, monoclonal |
| form【形式】 | buffered aqueous solution |
| species reactivity | human |
| concentration【浓度】 | ~1.0 mg/mL |
| technique(s) | ELISA: 0.1-0.2 μg/mL using 2.5 μg/mL Human IgM myeloma for coating immunoblotting: suitable |
| isotype【同位素/亚型】 | IgG2b |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
