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产品别名
Anti-HPK1 Antibody
Mitogen-activated protein kinase kinase kinase kinase 1, Hematopoietic progenitor kinase, MAPK/ERK kinase kinase kinase 1, MEK kinase kinase 1, MEKKK 1
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Hematopoietic progenitor kinase 1 (HPK1), otherwise known as MEKKK 1 and MAP4K1, is a serine/threonine kinase belonging to the Ste20 (Sterile-20) family. It is predominantly expressed in hematopoietic cells and is involved in sigaling pathways requiring MAP kinase activation; HPK1 directly upstream of MEKK1. HPK1 forms signaling complexes with a number of adaptor proteins including Nck, SLP-76, and Grb2 family of proteins; these proteins may facilitate the tethering of HPK1 to membrane receptors such as the epidermal growth factor receptor. However, HPK1 may also interact with adaptor proteins to induce transcription. For example, interaction with CRKL, and Crk adaptor proteins activates the c-Jun N-terminal kinase (JNK) pathway leading to gene expression. HPK1 may mediate cell growth, cytokine signaling, and cell death. |
| Specificity【特异性】 | Other homologies: Mouse and Rat (86% sequence homoloogy). This antibody recognizes HPK1 at the N-terminus. |
| Immunogen【免疫原】 | Epitope: N-terminus KLH-conjugated linear peptide corresponding to the N-terminus of human HPK1. |
| Application【应用】 | Anti-HPK1 Antibody detects level of HPK1 & has been published & validated for use in Western Blotting, IHC(P). Research Category Signaling Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected HPK1 in human tonsil tissue. Research Sub Category Developmental Signaling |
| Quality【质量】 | Evaluated by Western Blot in Raji cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected HPK1 on 10 µg of Raji cell lysate. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified Protein A purified |
| Analysis Note【分析说明】 | Control Raji cell lysate |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_009112 |
| UniProt accession no.【UniProt登记号】 | Q92918 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~97kDa observed. The calculated molecular weight is 91 kDa, however HPK1 has been shown as a ~97 kDa band in western blots. (Hu, M., et al. (1996). Genes Dev. 10:2251-2264.) |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
