Anti-HP1γ Antibody, clone 14D3.1

32160702

Chromobox protein homolog 3 (UniProt Q13185; also known as HECH, Heterochromatin protein 1 homolog gamma, HP1 gamma, Modifier 2 protein) is encoded by the CBX3 gene (Gene ID 11335) in human. HP1 is a non-histone chromosomal protein that is present as three orthologs, HP1-alpha, HP1-beta, and HP1-gamma, in mammals. While HP1-alpha and HP1-beta have been unambiguously localized in heterochromatin, HP1-gamma has been found to localize in both euchromatin and heterochromatin (transiently) compartments of interphase nuclei, as well as in the pericentromeric chromatin and arms of mitotic chromosomes of 3T3 cells. HP1-gamma is phosphorylated by PIM1 during interphase and may be hyper-phosphorylated during mitosis. HPI-gamma is involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at ′Lys-9′, leading to epigenetic repression. It may also contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). It is also reported to be involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Ref.: Minc E., et al. (2000). Cytogenet. Cell Genet. 90, 279-284.
Lachner, M., et al. (2001). Nature 410, 116-120.

KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human HP1γ.

Anti-HP1γ, clone 14D3.1, Cat. No. MABE656, is a highly specific mouse monoclonal antibody that targets heterochromatin protein-1 gamma and has been tested in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected nuclear HP1γ immunoreactivity in 4% paraformaldehyde-fixed, 0.1 Triton X-100-permeabilized A431 and HeLa cells.
Research Category
Epigenetics & Nuclear Function

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected HP1γ in 10 µg of HeLa cell lysate.

Replaces: MAB3450

Protein G purified.
Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified antibody

primary antibodies

14D3.1, monoclonal

human

mouse (based on 100% sequence homology), rat (based on 100% sequence homology), nonhuman primates (based on 100% sequence homology)

immunocytochemistry: suitable
western blot: suitable

IgG2aκ

NP_009207

Q13185

ambient

human ... CBX3(11335)

~20 kDa observed. 20.68 kDa(Met1 removed) calculated. Uncharacterized bands may be observed in some lysate(s).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1