Anti-HLCS Antibody, clone 2C2.1

32160702

NA.41

Biotin-protein ligase (EC 6.3.4.9; EC 6.3.4.10; EC 6.3.4.11; EC 6.3.4.15; UniProt P50747; also known as Biotin-[acetyl-CoA-carboxylase] ligase, Biotin apo-protein ligase, Biotin-[methylcrotonoyl-CoA-carboxylase] ligase, Biotin-[methylmalonyl-CoA-carboxytransferase] ligase, Biotin-[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase, HCS, Holocarboxylase synthetase) is encoded by the HLCS (also known as HCS) gene (Gene ID 3141) in human. HLCS (holocarboxylase synthetase) is the sole biotin protein ligase encoded by the human genome. HLCS catalyses the covalent modification of target lysine residues by biotin. In addiition to taregting various carboxylases in the cytoplasm and mitochondria, both the full-length HLCS and a splicing variant are reported to enter the nucleus and associate with multi-protein gene repression complex. HLCS overexpression in HEK293 cells is reported to downregulate histone H3 lysine9 acetylation (H3K9ac) level with a cocomitant drop in LTRs (long terminal repeats) 15 and 22 due to a decrease in mRNA coding for LTRs. HLCS is also reported to induce RANTES expression in HEK-293 cells by biotinylating heat shock protein 72 (HSP72).

GST-tagged recombinant protein corresponding to the N-terminal region of human HLCS.
Epitope: N-terminal region

Anti-HLCS Antibody, clone 2C2.1 is an antibody against HLCS for use in Western Blotting.
Research Sub Category
Metabolic Hormones & Receptors
Research Category
Signaling

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected HLCS in 10 µg of human skeletal muscle tissue lysate.

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified immunoglobulin

primary antibodies

2C2.1, monoclonal

human

western blot: suitable

IgG2aκ

NP_000402

P50747

wet ice

human ... HLCS(3141)

~80 kDa observed. Uncharacterized band(s) may appear in some lysates.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1