Anti-Histone H2A.Z Antibody (C-term)

32160702

H2A.Z, also known as H2AFZ, is a variant of histone H2A that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.

wide range expected
Recognizes histone H2A.Z

KLH-conjugated linear peptide corresponding to region the near C-terminus of Human Histone H2A.Z.

Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody (1μg per reaction) was used for ChIP using chromatin from HeLa and K562 cells.

ChIP-seq: A representative lot of this antibody (0.5 μg per reaction) was used to immunoprecipitate K562 chromatin for ChIP-seq analysis.

Western Blotting Analysis: A 1:2,000 dilution of a represenative lot of this antibody detected histone H2A.Z in acid extracted HeLa cell lysate.

Immunocytochemistry: A 1:500 dilution from a representative lot detected histone H2A.Z in HeLa cells.
This Anti-Histone H2A.Z Antibody (C-term) is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq and Immunocytochemistry and ELISA for the detection of Histone H2A.Z (C-term).

Evaluated by Western Blotting using recombinant histones

Western Blotting Analysis: A 1.5 µg/mL dilution of this antibody detected recombinant Histone H2A.Z, but not histones H1, H2A,H2A.X, H3, or H4.

Affinity purified rabbit polyclonal in PBS containing 0.05% azide and 0.05% ProClin 300.
Antigen Affinity Purified

Concentration: Please refer to lot specific datasheet.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human

ChIP: suitable (ChIP-seq)
ELISA: suitable
immunocytochemistry: suitable
western blot: suitable

NP_002097

P0C0S5

wet ice

human ... H2AFZ(3015)

~16 kDa observed

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

GHS07

Warning

H317

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Skin Sens. 1

12 - Non Combustible Liquids

WGK 2