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Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-1H5
| 产品编号： | 4108673 |
| 规格： | ascites fluid, clone 2HP-1H5, Chemicon® |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-1H5
HP1a, Antigen p25, Chromobox Protein Homolog 5
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated. |
| Specificity【特异性】 | Reacts with Heterochromatin protein-1 alpha (HP1alpha) between amino acids 67 and 119. |
| Immunogen【免疫原】 | Recombinant mouse HP1a. |
| Application【应用】 | Use Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-1H5 (mouse monoclonal antibody) validated in ELISA, ICC, IHC to detect Heterochromatin Protein-1 α also known as HP1a, Antigen p25, Chromobox Protein Homolog 5. Immunocytochemistry: 1:500-1:5,000 Immunohistochemistry: 1:500-1:5,000 ELISA: 1:500-1:5,000 Western blot: not recommended. For Western blot use MAB3446. Optimal working dilutions must be determined by the end user. |
| Physical form【外形】 | Unpurified Liquid ascites no preservative. |
| Analysis Note【分析说明】 | Control Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | ascites fluid |
| antibody product type | primary antibodies |
| clone【克隆】 | 2HP-1H5, monoclonal |
| species reactivity | human, mouse |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable immunocytochemistry: suitable immunohistochemistry: suitable |
| isotype【同位素/亚型】 | IgG1κ |
| suitability【适用性】 | not suitable for Western blot |
| NCBI accession no.【NCBI登记号】 | NM_012117.1 |
| UniProt accession no.【UniProt登记号】 | P45973 |
| shipped in【运输】 | dry ice |
| Gene Information | human ... CBX5(23468) |
产品说明
| 【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
