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Anti-Hec1 Antibody
| 产品编号： | 4108620 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 50 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-Hec1 Antibody
Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, HsHec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC, Hec1
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | The protein HEC1 (highly expressed in cancer 1) is an essential component of the vital NDC80 kinetochore-associated protein complex. The NDC80 complex is required for chromosome segregation and spindle checkpoint activity. HEC1 is essential for proper kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. HEC1 is expressed by all dividing cells and is particularly high in cancers. HEC1 localizes to kinetochores from late prophase to anaphase, specifically at the outer plate of the kinetochore. HEC1 is normally phosphorylated during its active phase at multiple sites and the phosphorylation appears to tune kinetochore-microtubule binding affinity during mitosis. Moreover, recent research using small molecule inhibitors to HEC1′s kinase, Nek2, demonstrate that phosphorylation is required for proper HEC1 function. |
| Immunogen【免疫原】 | Epitope: Nuclear localization region KLH-conjugated linear peptide corresponding to the Nuclear localization region of human Hec1. |
| Application【应用】 | Research Sub Category Nuclear Receptors Anti-Hec1 Antibody is an antibody against Hec1 Antibody for use in Western Blotting, Immunocytochemistry. Research Category Epigenetics & Nuclear Function Immunocytochemistry Analysis: 1.0 µg/mL from a representative lot detected Hec1 in HeLa cells. |
| Quality【质量】 | Evaluated by Western Blotting in SK-OV-3 cell lysate. Western Blotting Analysis: 0.1 µg/mL of this antibody detected Hec1 in 10 µg of SK-OV-3 cell lysate. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology), mouse (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_006092 |
| UniProt accession no.【UniProt登记号】 | O14777 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~78 kDa observed. Uncharacterized band(s) may appear in some lysates. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
