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Anti-HD-polyLeu-Ct

产品编号:4108602
规格:serum, from rabbit
包装规格:25 μL,100 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
410860225 μL1980
4108602100 μL4600
产品别名

Anti-HD-polyLeu-Ct

HD-RAN PolyLeu, HD-PolyLeu

基本信息
eCl@ss
32160702
General description【一般描述】
Huntington disease (HD) is a progressive neurodegenerative disorder is caused by a CAG,CTG expansion in the HTT gene that results in the production of a mutant huntingtin protein (HTT) with polymeric expansions that accumulate in human brain with HD. PolyLeu is an antisense repeat-associated non-ATG (RAN) translation protein that accumulate in various brain regions with toxic effects leading to microglial activation and neuronal loss. HD-RAN polyLeu toxicity is shown to be comparable to that of polyGln. HD-RAN proteins are abundant in regions of the brain that are most affected by HD and these regions display pathologic features of HD, such as caspase-3 activity and microglial activation. HD-Ran protein accumulation and aggregation in HD brains are shown to be length dependent.
Specificity【特异性】
This rabbit polyclonal antibody specifically detects RAN proteins with PolyLeu-HD.
Immunogen【免疫原】
A synthetic peptide corresponding to the C-terminal region of the predicted polyLeu frames of HD in the CTG direction (HD-Leu-Ct).

Epitope: unknown
Application【应用】
Anti-HD-polyLeu-Ct, Cat. No. ABN1670, is a highly specific rabbit polyclonal antibody that targets HD-polyLeu-Ct and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Research Category
Neuroscience
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected HD-polyLeu-Ct in HEK293T cells transfected with HD-polyLeu-Ct.

Immunocytochemistry Analysis: A representative lot detected HD-polyLeu-Ct in Immunocytochemistry applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).

Western Blotting Analysis: A representative lot detected HD-polyLeu-Ct in Western Blotting applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).

Immunohistochemistry Analysis: A representative lot detected HD-polyLeu-Ct in Immunohistochemistry applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).
Quality【质量】
Evaluated by Western Blotting in HEK293T cells transfected with HD-polyLeu-Ct.

Western Blotting Analysis (WB): A 1:2,000 dilution of this antibody detected PolyLeu in HEK293T cells transfected with HD-polyLeu-Ct, but not in control cells.





Physical form【外形】
Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide.
Other Notes【其他说明】
Concentration: Please refer to lot specific datasheet.
产品性质
biological source【生物来源】
rabbit
antibody form【抗体形式】
serum
antibody product type
primary antibodies
clone【克隆】
polyclonal
species reactivity
human
packaging【包装】
antibody small pack of 25 μL
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype【同位素/亚型】
IgG
产品说明
Target description【目标描述】
~21 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Storage and Stability【储存及稳定性】
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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