Anti-Girdin, coiled-coil region Antibody

32160702

NA.41

Girdin (Ccdc88a; APE, GIV, or HkRP1) is a ubiquitous protein belonging to the CCDC88 family. Girdin is an actin-binding protein which interacts with actin via its C-terminal domain, and may play a crucial role in cell motility and cytoskeleton remodeling. These effects may be regulated by a reciprocal relationship between Girdin and Akt. Akt phosphorylates Girdin, and Girdin binds to Akt and enhances phosphorylation of Akt, thereby promoting phosphorylation of Akt’s downstream targets such as GSK-3β and FOXO1A. Girdin/Akt signaling may play an important role in cancer by facilitating the proliferation of cancer cells.

Other Homologies: Mouse (97% sequence homology).

GST-tagged recombinant protein corresponding to the coiled coil domain of rat Girdin.

This Anti-Girdin, coiled-coil region Antibody is validated for use in ICC, Western Blotting, Immunoprecipitation for the detection of Girdin, coiled-coil region.
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected Girdin in Cos-7 cells (Prof. P. Ghosh, Department of Medicine, Division of Gastroenterology and Hepatology, UCSD School of Medicine and Veterans Affairs Medical Center).

Western Blot Analysis: A representative lot detected Girdin in Cos-7 cell lysate. Uncharacterized bands may be observed in some cell lysates.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Girdin in HeLa cell lysates (Prof. P. Ghosh, Department of Medicine, Division of Gastroenterology and Hepatology, UCSD School of Medicine and Veterans Affairs Medical Center). Uncharacterized bands may be observed in some cell lysates.

Immunocytochemistry Analysis: A 1:1000 dilution of this antibody detected Girdin in HeLa cells (Prof. A. Ghosh, Division of Biology, UCSD).

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Control
HeLa cells

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human, monkey

rat

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

XP_223709

wet ice

~250 kDa observed. Some uncharacterized bands may be observed in some cell lysates.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1