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Anti-Girdin Antibody
产品编号: | 4108155 |
规格: | from rabbit, purified by affinity chromatography |
包装规格: | 100 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Girdin (Ccdc88a; APE, GIV, or HkRP1) is a ubiquitous protein belonging to the CCDC88 family. Girdin is an actin-binding protein which interacts with actin via its C-terminal domain, and may play a crucial role in cell motility and cytoskeleton remodeling. These effects may be regulated by a reciprocal relationship between Girdin and Akt. Akt phosphorylates Girdin, and Girdin binds to Akt and enhances phosphorylation of Akt, thereby promoting phosphorylation of Akt’s downstream targets such as GSK-3β and FOXO1A. Girdin/Akt signaling may play an important role in cancer by facilitating the proliferation of cancer cells. |
Specificity【特异性】 | This antibody recognizes the coiled-coil domain of Girdin. Other homologies: Mouse (97% sequence homology). |
Immunogen【免疫原】 | Epitope: Coiled coil domain. GST-tagged recombinant protein corresponding to the coiled coil domain rat Girdin. |
Application【应用】 | Research Category Cell Structure This Girdin antibody is validated for use in WB, IHC, IF & IP for the detection of the Girdin protein. Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Girdin in human colorectal cancer tissues. Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Girdin in HeLa, Cos-7, and Att20 cells. Immunoprecipitation Analysis: A representative lot immunoprecipitated Girdin in HeLa cell lysate. Research Sub Category Cytoskeleton |
Quality【质量】 | Evaluated by Western Blotting in Cos-7 cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Girdin in 10 µg of Cos-7 cell lysate. |
Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
Analysis Note【分析说明】 | Control Cos-7 cell lysate |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | human, monkey |
species reactivity (predicted by homology) | rat (immunogen homology) |
technique(s) | immunofluorescence: suitable immunohistochemistry: suitable (paraffin) immunoprecipitation (IP): suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | XP_223709 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | ~220 kDa observed. Uncharacterized band(s) may be observed in some cell lysates. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |