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Anti-GABA Antibody

产品编号:4108011
规格:serum, Chemicon®
包装规格:50 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
410801150 μL6690
基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system that inhibits the generation of the action potential of the neuron. It is involved in the pathogenesis of certain neurological and psychiatric disorders. GABA is produced from glutamic acid in a reaction catalyzed by glutamic acid decarboxylase. In this reaction pyridoxal phosphate serves as a co-factor. GABA interacts with the GABAA and GABAB receptors, which are widely distributed throughout the nervous system and in a variety of cell types. They differ in their pharmacological, electrophysiological, and biochemical properties. GABAA-receptor complex mediates an increase in membrane conductance with an equilibrium potential near the resting level of −70 mV. This conductance increase often is accompanied by a membrane hyperpolarization, which later results in a reduction in the probability of action potential initiation. The reduction in membrane resistance is accomplished by the GABA-dependent facilitation of Cl− ion influx. GABAB receptors are coupled indirectly to K+ channels. When activated, GABAB receptors reduce Calcium ion conductance and inhibit cAMP production via intracellular mechanisms mediated by G-proteins. GABAB receptors are known to mediate both postsynaptic and presynaptic inhibition.
Specificity【特异性】
GABA is a neurotransmitter synthesized in all species, and is therefore expected to react to all species.
Recognizes GABA. Staining was blocked by preabsorbing with 100 μM GABA conjugated to glutaraldehyde. 500 μM of similar conjugations of glutamic acid, glutamate and taurine failed to block staining.
Immunogen【免疫原】
BSA-conjugated GABA-Glutaraldehyde
Application【应用】
Research Category
Neuroscience
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected GABA in rat brain tissue.

ELISA Analysis: A representative lot from an independent laboratory detected GABA in a competitive ELISA.

Immunohistochemistry Protocol:

Tissues fixed with 4% paraformaldehyde and 0-0.5% glutaraldehyde gives good results. Glutaraldehyde is required for antibody reactivity.

1) Tissue is fixed with 4% paraformaldehyde, 0-0.5% glutaraldehyde, 0.5% potassium dichromate in 0.1M phosphate buffer at pH 6.5.

2) Tissue is post-fixed overnight, vibratome sectioned in 50 mm and incubated in 0.05M Tris buffer, pH 6.5 for three hours.

3) Sections are incubated for 18-24 hours in AB175 diluted in PBS containing 0.1% sodium azide, 0.2% Triton X-100 and 1% normal goat serum.

4) Fluorescein conjugated antibody or ABC system may be used as the secondary reagent.

Note: Without colchicine pretreatment well-stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchicine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei.
Research Sub Category
Neurotransmitters & Receptors
This Anti-GABA Antibody is validated for use in Immunohistochemistry (Paraffin) and Enzyme Immunoassay (ELISA) for the detection of GABA.
Quality【质量】
Evaluated by Immunohistochemistry in rat brain tissue.

Immunohistochemistry Analysis: A 1:500 dilution of this antibody detected GABA in rat brain tissue.
Physical form【外形】
Depleted serum
Guinea Pig polyclonal BSA-depleted antiserum with 0.05% sodium azide.
Analysis Note【分析说明】
Control
Rat Brain tissue
Other Notes【其他说明】
Concentration: Variable
Legal Information【法律信息】
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
guinea pig
antibody form【抗体形式】
serum
antibody product type
primary antibodies
clone【克隆】
polyclonal
species reactivity
rat
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable (paraffin)
shipped in【运输】
dry ice
产品说明
Storage and Stability【储存及稳定性】
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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