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Anti-Folic acid antibody, Mouse monoclonal

产品编号:4107884
规格:clone VP-52, purified from hybridoma cell culture
包装规格:100 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4107884100 μL6330
产品别名

Anti-Folic acid antibody, Mouse monoclonal

Anti-vitamin B9

Anti-vitamin M

Anti-FA

Anti-pteroyl-L-glutamate

Anti-pteroyl-L-glutamic acid

Anti-folacin

Anti-N-(4-{[(2-amino-4-oxo-1,4-dihydropteridin-6-yl)methyl]amino}benzoyl)-L-glutamic acid

Anti-folate

Anti-vitamin Bc

产品性质
Quality Level【质量水平】
200
biological source【生物来源】
mouse
antibody form【抗体形式】
purified from hybridoma cell culture
antibody product type
primary antibodies
clone【克隆】
VP-52, monoclonal
form【形式】
buffered aqueous solution
concentration【浓度】
~1 mg/mL
technique(s)
immunoblotting: suitable
indirect ELISA: 0.25-0.5 μg/mL using 3 μg/mL folic acid conjugated to a carrier for coating
isotype【同位素/亚型】
IgG2b
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
基本信息
General description【一般描述】
Folic acid (pteroylglutamic acid) and Vitamin B12 (cobalamin) are essential constituents for normal growth of mammalian cells. The normal serum range is 3-16 ng/ml for folate and 0.2-0.9 ng/ml for vitamin B12. Folic acid plays an important role in cellular metabolic activities such as functioning as a cofactor in the one-carbon metabolism for DNA and RNA synthesis as well as nucleotide and amino acid biosynthesis. A lack of folate or folic acid nutrition can lead to folic acid deficiency and result in several health problems, including macrocytic anemia, elevated plasma homocysteine, cardiovascular disease, birth defects, carcinogenesis, muscle weakness and walking difficulty.2 These deficiencies may appear in pregnant women, alcoholics, those whose diets do not include raw fruits and vegetables and people with structural or functional damage to the small intestine. Vitamin B12 and folic acid are metabolically interrelated.

In the absence of vitamin B12, 5MTHFA cannot be converted to tetrahydrofolic acid and enter the metabolic pool of 1-carbon fragment acceptors. Since this is the only known metabolic pathway involving 5MTHFA in humans, B12 depletion will cause a decrease in the availability of other folic acid derivatives required for miscellaneous biosynthetic pathways, including thymidylate synthetase, an enzyme necessary for DNA synthesis. Vitamin B12 and folate deficiencies are the most common causes of megaloblastic anemia, abnormal hemopoiesis, interference in the maintenance of normal nerve tissue and general intracellular uptake and function disorders in humans. It is hematologically and clinically indistinguishable thus it is necessary to determine the level of vitamin B12 in the serum and folate in both the serum and red blood cells to establish the etiology of the megaloblastic anemia and treatment respectively.

Monoclonal Anti-Folic acid recognizes folate and an epitope present on both biologically active analog 5MTHFA and folic acid. The antibody reacts with Folic acid or 5MTHFA either free or bound to a carrier, such as KLH or BSA. The antibody binds folate in human plasma and serum (when it is bound to the endogenous folate binder). The antibody does not cross react with tetrahydrofolic (THFA), folinic acid (FNA), dihydrofolic acid (FAH2, citrovorum factor, leucovorin).
Immunogen【免疫原】
5-Methyltetrahydrofolic acid (5MTHFA) conjugated to KLH
Application【应用】
The antibody is recommended to use in various immunological techniques, including ELISA and Immunobloting.
Physical form【外形】
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Other Notes【其他说明】
This product is for R&D use only, not for drug, household, or other uses.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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