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Anti-FBXO15 Antibody, clone 3B11.1

产品编号:4107770
规格:clone 3B11.1, from mouse
包装规格:200 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4107770200 μL5350
产品别名

Anti-FBXO15 Antibody, clone 3B11.1

F-box only protein 15

基本信息
eCl@ss
32160702
General description【一般描述】
F-box only protein 15 (UniProt Q8NCQ5) is encoded by the FBXO15 (also known as FBX15) gene (Gene ID 201456) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, U-box-type, and PHD-finger-type E3 ligases. The RING finger-type E3s include seven cullin-based E3s, such as SKP1–CUL1–F-box-protein (SCF) complex and the anaphase-promoting complex/cyclosome (APC/C). F-box proteins use their N-terminal F-box motif to bind Skp1 and other motif(s) in their C-terminal region for substrates recognition and recruitment. F-box proteins are divided into the FBXW subclass that contains WD40 repeat domains, the FBXL subclass that contains leucine-rich repeat domains, and the F-box only (FBXO) subclass that contains not yet fully characterized substrate recognition domains. SCF-Fbxo15 is reported to mediate the ubiquitination of cardiolipin synthase (CLS1) for proteasomal degradation in human lung epithelial cells. PTEN-induced putative kinase 1 (PINK1) is shown to phosphorylate CLS1 and cause its release from the inner mitochondrial membrane to the outer membrane, making it accessible for Fbxo15-targeted ubiquitination.
Specificity【特异性】
Clone 3B11.1 recoginzes an N-terminal epitope present in spliced isoform 1 (UniProt Q8NCQ5-1), but absent in spliced isoform 2 (UniProt Q8NCQ5-2).
Immunogen【免疫原】
KLH-conjugated linear peptide corresponding an N-terminal sequence of human FBXO15.
Epitope: N-terminal region.
Application【应用】
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
Research Category
Stem Cell Research
Anti-FBXO15 Antibody, clone 3B11.1 is an antibody against FBXO15 for use in Western Blotting.
Quality【质量】
Evaluated by Western Blotting in human H9 embryonic stem cell lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected FBXO15 in 10 µg of human H9 embryonic stem cell lysate.
Physical form【外形】
Purified mouse monoclonal IgMκ antibody in buffer containing PBS with 0.05% sodium azide.
Purified by Ion-Exchange Chromatography
Format: Purified
Other Notes【其他说明】
Concentration: Please refer to lot specific datasheet.
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
3B11.1, monoclonal
species reactivity
human
technique(s)
western blot: suitable
isotype【同位素/亚型】
IgMκ
NCBI accession no.【NCBI登记号】
NP_001136430
UniProt accession no.【UniProt登记号】
Q8NCQ5
产品说明
Target description【目标描述】
~57 kDa observed. 57.26 kDa calculated. Uncharacterized band(s) may appear in some lysates.
Storage and Stability【储存及稳定性】
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 2
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