Anti-FANCA Antibody, clone 5G9

32160702

NA.41

FANCA, also named as FAA, FACA and FANCH, belongs to the multisubunit FA complex which is not found in patients with Fanconi anemia. FANCA may be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Mutations in FANCA have been associated with Fanconi anemia (FA) which is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies.

KLH-conjugated linear peptide corresponding to the C-terminus of human FANCA.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected FANCA in 10 µg of HeLa cell lysate.

Western Blotting Analysis: A representative lot from an independent laboratory detected FANCA in normal HSC93 and FA mutant lymphoblast whole cell extracts. The same lot also detected FANCA in HeLa whole cell extracts and in FANCA transfected PD430 whole cell extracts (Tremblay, C. S., et al. (2008). Blood. 112(5):2062-2070.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated FANCA from yeast extracts expressing FANCA. The same lot also immunoprecipiated FANCA from 293T whole cell extracts expressing FANCA (Tremblay, C. S., et al. (2008). Blood. 112(5):2062-2070.)

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated FANCA from FANCA wild type FA lymphoblast cell lysate (de Winter, J. P., et al. (2000). Hum Mol Genet. 9(18):2665-2674.).
Anti-FANCA Antibody, clone 5G9 is a highly specific mouse monoclonal antibody, that targets FANCA & has been tested in western blotting & IP.

Evaluated by Western Blotting in Raji cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FANCA in 10 µg of Raji cell lysate.

Protein G Purified
Format: Purified

100

mouse

purified immunoglobulin

primary antibodies

5G9, monoclonal

human

immunoprecipitation (IP): suitable
western blot: suitable

IgG1κ

NP_000126

O15360

wet ice

human ... FANCA(2175)

~165 kDa observed

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12 - Non Combustible Liquids

WGK 1