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Anti-EB1, EB2 and EB3 Antibody, clone KT65
产品别名
Anti-EB1, EB2 and EB3 Antibody, clone KT65
Microtubule-associated protein RP/EB family member 1, APC-binding protein EB1, End-binding protein 1, EB1, EB2, End binding protein 2, EB3, End binding protein 3, Microtubule-associated protein RP/EB family member 3
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | EB (End-binding) proteins are evolutionarily conserved and are important for extending tips of microtubules as well as regulating microtubule dynamics and communication with other intracellular substances. EB1 (End binding protein 1) encoded by the gene EB1/MAPRE1, EB2 (End binding protein 2) encoded by EB2/MAPRE2, and EB3 (End binding protein 3 also known as Microtubule-associated protein RP/EB family member 3) encoded by EB3/RP3/EBF3-S2/EBF3/MAPRE3 bind directly with end-tracking proteins (+TIPs). +TIPs influence microtubule growth, rescue, dynamicity, and suppression. EB1, EB2, and EB3 play major roles in cellular migration and adhesion, and are thought to associate with adenomatous polyposis coli tumor suppressor. |
Specificity【特异性】 | This antibody is expected to detect EB1, EB2 and EB3. |
Immunogen【免疫原】 | GST-tagged recombinant protein corresponding to mouse EB1, EB2, and EB3. |
Application【应用】 | Research Category Cell Structure Research Sub Category Cytoskeletal Signaling Immunocytochemistry Analysis: A representative lot detected EB1, EB2, and EB3 in NIH/3T3 cells (Van der Vaart, B., et al. (2011). J Cell Biol. 193(6):1083-1099). Immunoprecipitation Analysis: A representative lot immunoprecipitated EB1, EB2, and EB3 in HeLa cell extracts (Van der Vaart, B., et al. (2011). J Cell Biol. 193(6):1083-1099). This Anti-EB1, EB2 & EB3 Antibody, clone KT65 is validated for use in western blotting, ICC & IP for the detection of EB1. |
Quality【质量】 | Evaluated by Western Blotting in MAPRE1 (EB1), MAPRE2 (EB2), and MAPRE3 (EB3) cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected EB1, EB2, and EB3 in 10 µg of MAPRE1 (EB1), MAPRE2 (EB2), and MAPRE3 (EB3) cell lysate. |
Physical form【外形】 | Purified rat monoclonal IgG2aκ in buffer containing PBS. Format: Purified Protein G Purified |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | rat |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | KT65, monoclonal |
species reactivity | mouse, human |
technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG2aκ |
NCBI accession no.【NCBI登记号】 | NP_031922 |
UniProt accession no.【UniProt登记号】 | Q61166 Q6PER3 Q8R001 |
shipped in【运输】 | wet ice |
Gene Information | human ... EBF3(253738), MAPRE2(10982), MAPRE3(22924) |
产品说明
Target description【目标描述】 | 31, 37, 32 kDa calculated. The calculated MW of EB1 (31 kDa), EB2 (37 kDa), and EB3 (32 kDa). The recombinants used to test the reactivity of this antibody to EB1, EB2, and EB3 have the following MW′s. EB1 (56 kDa), EB2 (62 kDa), and EB3 (58 kDa). |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 2 |