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Anti-E3 ubiquitin-protein ligase UHRF1 Antibody, clone 7C8
产品别名
Anti-E3 ubiquitin-protein ligase UHRF1 Antibody, clone 7C8
E3 ubiquitin-protein ligase UHRF1, Inverted CCAAT box-binding protein of 90 kDa, Nuclear protein 95, Nuclear zinc finger protein Np95, HuNp95, hNp95, RING finger protein 106, Transcription factor ICBP90, Ubiquitin-like PHD and RING finger domain-containi
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Ubiquitin-like with PHD and ring finger domains 1 (UHRF1), also known as ICBP90 or Np95, is a multifunctional nuclear protein that may be an important mediator of heterochromatin formation which is indicative of gene repression. UHRF1 contains a set and RING-finger-associated domain that detects hemi-methylated cytosine residues leading to the recruitment of DNMT1 to these residues during the S phase. UHRF1 thereby facilitates the transfer of methylation status to newly synthesized DNA molecules. UHRF1 may also interact with histones and HDAC1 via its PHD domain to promote modifications of histone residues which contribute to heterochromatin formation. UHRF1 may also directly modify histone H3 by ubiquitination. In addition, UHRF1 may contribute to the DNA-repair process though associations with PCNA. Several studies have suggested that overexpression of UHRF1 may contribute to the development of many cancers. |
| Immunogen【免疫原】 | Recombinant protein corresponding to human E3 ubiquitin-protein ligase UHRF1. |
| Application【应用】 | Research Sub Category Nuclear Receptors Western Blotting Analysis: A representative lot detected E3 ubiquitin-protein ligase UHRF1 in overexpressed lysate (Martin Walsh, Mount Sinai School of Medicine). Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated E3 ubiquitin-protein ligase UHRF1 in NTera2 cells (Martin Walsh, Mount Sinai School of Medicine). This Anti-E3 ubiquitin-protein ligase UHRF1 Antibody, clone 7C8 is validated for use in Immunocytochemistry and Chromatin Immunoprecipitation (ChIP) and Western Blotting for the detection of E3 ubiquitin-protein ligase UHRF1. Research Category Epigenetics & Nuclear Function |
| Quality【质量】 | Evaluated by Immunocytochemistry in HeLa cells. Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected E3 ubiquitin-protein ligase UHRF1 in HeLa cells. Alexa Fluor® is a registered trademark of Life Technologies |
| Physical form【外形】 | Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified Protein G Purified |
| Legal Information【法律信息】 | ALEXA FLUOR is a registered trademark of Life Technologies |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | 7C8, monoclonal |
| species reactivity | human |
| concentration【浓度】 | 1 mg/mL |
| technique(s) | ChIP: suitable immunocytochemistry: suitable western blot: suitable |
| isotype【同位素/亚型】 | IgG2bκ |
| NCBI accession no.【NCBI登记号】 | NP_001041666 |
| UniProt accession no.【UniProt登记号】 | Q96T88 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | 90 kDa calculated |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
