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Anti-E3 ubiquitin-protein ligase UHRF1 Antibody
| 产品编号： | 4107279 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-E3 ubiquitin-protein ligase UHRF1 Antibody
E3 ubiquitin-protein ligase UHRF1, Inverted CCAAT box-binding protein of 90 kDa, Nuclear protein 95, Nuclear zinc finger protein Np95, HuNp95, hNp95, RING finger protein 106, Transcription factor ICBP90, Ubiquitin-like PHD and RING finger domain-containi
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Ubiquitin-like with PHD and ring finger domains 1 (UHRF1), also known as ICBP90 or Np95, is a multifunctional nuclear protein that may be an important mediator of heterochromatin formation which is indicative of gene repression. UHRF1 contains a set and RING-finger-associated domain that detects hemi-methylated cytosine residues leading to the recruitment of DNMT1 to these residues during the S phase. UHRF1 thereby facilitates the transfer of methylation status to newly synthesized DNA molecules. UHRF1 may also interact with histones and HDAC1 via its PHD domain to promote modifications of histone residues which contribute to heterochromatin formation. UHRF1 may also directly modify histone H3 by ubiquitination. In addition, UHRF1 may contribute to the DNA-repair process though associations with PCNA. Several studies have suggested that overexpression of UHRF1 may contribute to the development of many cancers. |
| Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to a region near the C-terminus of human E3 ubiquitin-protein ligase UHRF1. |
| Application【应用】 | Detect UHRF1 using this rabbit polyclonal antibody, Anti-E3 ubiquitin-protein ligase UHRF1 Antibody validated for use in western blotting & IHC. Research Category Epigenetics & Nuclear Function Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected E3 ubiquitin-protein ligase UHRF1 in human breast cancer tissue. Research Sub Category Chromatin Biology |
| Quality【质量】 | Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected E3 ubiquitin-protein ligase UHRF1 in 10 µg of HEK293 cell lysate. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | primate (based on 100% sequence homology), feline (based on 100% sequence homology), porcine (based on 100% sequence homology), canine (based on 100% sequence homology), bovine (based on 100% sequence homology), horse (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_001041666.1 |
| UniProt accession no.【UniProt登记号】 | Q96T88 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~97 kDa observed |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
