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Anti-Dopamine D2 Receptor Antibody

产品编号:4107173
规格:serum, Chemicon®
包装规格:50 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
410717350 μL5520
基本信息
eCl@ss
32160702
NACRES
NA.41
Specificity【特异性】
Recognizes Dopamine D2 Receptor (D2R). Recognizes both native and denatured forms of D2R (110, 68 and 47 kDa bands by Western blot using rat striatal positive control (Catalog Number AG282)). AB1558 inhibits binding of [3H]YM-09152-2, a known D2R antagonist and (125)I-NAPS, a photoligand. It inhibits binding of [3H]YM-09152-2 and PPHT-fluorescein (an agonist of D2R) and reacts with 110 kDa D2R band in fibroblasts transfected with rat D2R cDNA. AG284 is available for blocking experiments; AG284 is not recommended as a positive control for western blots.
Immunogen【免疫原】
Amino-terminal peptide sequence (aa 24-34) near or at the ligand binding domain of rat D2R (1,2).

The immunogen shows 100% homology with mouse, 90% with human and bovine and 72% with canine D2R receptors. An antibody made to the same sequence has shown reactivity with human, lobster, squid and crawfish.
Application【应用】


Immunohistochemistry: 4% PFA fixed, frozen sections, 1:200, 24 hour incubation at room temperature, DAB detection. {Tillerson, JL et al. (2006) Behavioural Brain Res. 172:97-105.

Immunocytochemistry: 4% PFA fixed cells 10-15′, permeabilized with 0.2-0.3% triton X-100, 1:100, o/N 4C for fluorescence {Sun, W et al (2005) Stem Cells 23(7):931-945, http://stemcells.alphamedpress.org/cgi/content/full/23/7/931}.

Western blot: 1:1,000-1:5,000 (Chemiluminescence technique) Higher concentration of antibody may be necessary when using low concentration samples or when using milk in antibody diluents. It may be necessary to load 100-200 μg of purified striatal or other tissue membranes to visualize the DR2 bands. The antibody has been shown to recognize both native and denatured DR2 bands.

ELISA: 1:10,000-1:50,000 [1 μg/mL D2R peptide (Cat. Number AG284)/well]

Optimal working dilutions must be determined by end user.
Anti-Dopamine D2 Receptor Antibody is an antibody against Dopamine D2 Receptor for use in ELISA, IC, IH & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Physical form【外形】
Unpurified
Rabbit antiSerum. Liquid containing 0.05% sodium azide.
Analysis Note【分析说明】
Control
Brain
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information【法律信息】
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
serum
antibody product type
primary antibodies
clone【克隆】
polyclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.【NCBI登记号】
NM_000795.2
NM_016574.2
UniProt accession no.【UniProt登记号】
P14416
shipped in【运输】
dry ice
产品说明
Target description【目标描述】
110, 68 and 47 kDa
Storage and Stability【储存及稳定性】
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 1
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