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Anti-Dopamine D2 Receptor Antibody
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
Specificity【特异性】 | Recognizes Dopamine D2 Receptor (D2R). Recognizes both native and denatured forms of D2R (110, 68 and 47 kDa bands by Western blot using rat striatal positive control (Catalog Number AG282)). AB1558 inhibits binding of [3H]YM-09152-2, a known D2R antagonist and (125)I-NAPS, a photoligand. It inhibits binding of [3H]YM-09152-2 and PPHT-fluorescein (an agonist of D2R) and reacts with 110 kDa D2R band in fibroblasts transfected with rat D2R cDNA. AG284 is available for blocking experiments; AG284 is not recommended as a positive control for western blots. |
Immunogen【免疫原】 | Amino-terminal peptide sequence (aa 24-34) near or at the ligand binding domain of rat D2R (1,2). The immunogen shows 100% homology with mouse, 90% with human and bovine and 72% with canine D2R receptors. An antibody made to the same sequence has shown reactivity with human, lobster, squid and crawfish. |
Application【应用】 | Immunohistochemistry: 4% PFA fixed, frozen sections, 1:200, 24 hour incubation at room temperature, DAB detection. {Tillerson, JL et al. (2006) Behavioural Brain Res. 172:97-105. Immunocytochemistry: 4% PFA fixed cells 10-15′, permeabilized with 0.2-0.3% triton X-100, 1:100, o/N 4C for fluorescence {Sun, W et al (2005) Stem Cells 23(7):931-945, http://stemcells.alphamedpress.org/cgi/content/full/23/7/931}. Western blot: 1:1,000-1:5,000 (Chemiluminescence technique) Higher concentration of antibody may be necessary when using low concentration samples or when using milk in antibody diluents. It may be necessary to load 100-200 μg of purified striatal or other tissue membranes to visualize the DR2 bands. The antibody has been shown to recognize both native and denatured DR2 bands. ELISA: 1:10,000-1:50,000 [1 μg/mL D2R peptide (Cat. Number AG284)/well] Optimal working dilutions must be determined by end user. Anti-Dopamine D2 Receptor Antibody is an antibody against Dopamine D2 Receptor for use in ELISA, IC, IH & WB. Research Category Neuroscience Research Sub Category Neurotransmitters & Receptors |
Physical form【外形】 | Unpurified Rabbit antiSerum. Liquid containing 0.05% sodium azide. |
Analysis Note【分析说明】 | Control Brain |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | serum |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
species reactivity | rat |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NM_000795.2 NM_016574.2 |
UniProt accession no.【UniProt登记号】 | P14416 |
shipped in【运输】 | dry ice |
产品说明
Target description【目标描述】 | 110, 68 and 47 kDa |
Storage and Stability【储存及稳定性】 | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |