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Anti-Dnmt1 Antibody, clone DNM-2C1
产品别名
Anti-Dnmt1 Antibody, clone DNM-2C1
DNA (cytosine-5)-methyltransferase 1, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA Mtase Hsal, M. Hsal, MCMT
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | DNA (cytosine-5)-methyltransferase 1 (Dnmt1) is one of three mammalian enzymes that catalyze the addition of a methyl group to the 5’ carbon of cytosine residues in CpG dinucleotides. These methylation marks are heritable modifications that are transferred to new DNA molecules following replication, by the activity of Dnmt1, and possibly Dnmt3a and Dnmt3b. Dnmt1 recognizes hemimethylated CpG regions with the aid of the UHRF1 protein, and it mediates the methylation of cytosines at those sites. DNA methylation has been linked to chromosome stability and gene expression in somatic cells; and to parental imprinting and X-chromosome inactivation in germ cells. Several studies have indicated that DNA methylation patterns are altered in various cancers. |
Immunogen【免疫原】 | His-tagged recombinant protein corresponding to human Dnmt1. |
Application【应用】 | Anti-Dnmt1 Antibody, clone DNM-2C1 is a Rat Monoclonal Antibody for detection of Dnmt1 also known as DNA (cytosine-5)-methyltransferase 1 & has been validated in IP, ChIP & WB. Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect Dnmt1 in IP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19):8355-8365.). Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate Dnmt1 in ChIP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19):8355-8365.). Research Category Epigenetics & Nuclear Function Research Sub Category Chromatin Biology |
Quality【质量】 | Evaluated by Western Blot in HeLa nuclear extract. Western Blot Analysis: 0.02 µg/mL of this antibody detected Dnmt1 in 10 µg of HeLa nuclear extract. |
Physical form【外形】 | Format: Purified Purified rat monoclonal IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Protein G Purified |
Analysis Note【分析说明】 | Control HeLa nuclear extract |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | rat |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | DNM-2C1, monoclonal |
species reactivity | human |
technique(s) | ChIP: suitable immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG2b |
NCBI accession no.【NCBI登记号】 | NP_001124295 |
UniProt accession no.【UniProt登记号】 | P26358 |
shipped in【运输】 | wet ice |
Gene Information | human ... DNMT1(1786) |
产品说明
Target description【目标描述】 | ~183 kDa observed. Three isoforms are known to exist at ~183 kDa, ~185 kDa, and ~145 kDa An uncharacterized band may be observed at ~90 kDa in some nuclear extracts. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |