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Anti-Dnmt1 Antibody, clone DNM-2C1

产品编号:4107137
规格:clone DNM-2C1, from rat
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4107137100 μG6020
产品别名

Anti-Dnmt1 Antibody, clone DNM-2C1

DNA (cytosine-5)-methyltransferase 1, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA Mtase Hsal, M. Hsal, MCMT

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
DNA (cytosine-5)-methyltransferase 1 (Dnmt1) is one of three mammalian enzymes that catalyze the addition of a methyl group to the 5’ carbon of cytosine residues in CpG dinucleotides. These methylation marks are heritable modifications that are transferred to new DNA molecules following replication, by the activity of Dnmt1, and possibly Dnmt3a and Dnmt3b. Dnmt1 recognizes hemimethylated CpG regions with the aid of the UHRF1 protein, and it mediates the methylation of cytosines at those sites. DNA methylation has been linked to chromosome stability and gene expression in somatic cells; and to parental imprinting and X-chromosome inactivation in germ cells. Several studies have indicated that DNA methylation patterns are altered in various cancers.
Immunogen【免疫原】
His-tagged recombinant protein corresponding to human Dnmt1.
Application【应用】
Anti-Dnmt1 Antibody, clone DNM-2C1 is a Rat Monoclonal Antibody for detection of Dnmt1 also known as DNA (cytosine-5)-methyltransferase 1 & has been validated in IP, ChIP & WB.
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect Dnmt1 in IP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19):8355-8365.).

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate Dnmt1 in ChIP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19):8355-8365.).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Quality【质量】
Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 0.02 µg/mL of this antibody detected Dnmt1 in 10 µg of HeLa nuclear extract.
Physical form【外形】
Format: Purified
Purified rat monoclonal IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified
Analysis Note【分析说明】
Control
HeLa nuclear extract
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
rat
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
DNM-2C1, monoclonal
species reactivity
human
technique(s)
ChIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype【同位素/亚型】
IgG2b
NCBI accession no.【NCBI登记号】
NP_001124295
UniProt accession no.【UniProt登记号】
P26358
shipped in【运输】
wet ice
Gene Information
human ... DNMT1(1786)
产品说明
Target description【目标描述】
~183 kDa observed. Three isoforms are known to exist at ~183 kDa, ~185 kDa, and ~145 kDa An uncharacterized band may be observed at ~90 kDa in some nuclear extracts.
Storage and Stability【储存及稳定性】
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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