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Anti-ATPIF1 Antibody
| 产品编号： | 4105215 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-ATPIF1 Antibody
ATPase inhibitor, mitochondrial, Inhibitor of F(1)F(o)-ATPase, IF(1), IF1, ATPIF1
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | ATPIF1 is an interesting protein that acts on ATP synthase and prevents it from catabolizing ATP when the mitochondrial membrane potential falls below a threshold, which ATP synthase starts to do by hydrolyzing ATP to pump protons out of the mitochondrial matrix to restore ion balance. ATPIF1 also indirectly regulates heme synthesis by modulating the mitochondrial pH and redox potential. ATPIF1 is expressed in the mitochondria and its active is determined by conformation. Homodimers are active, homotetramers are inactive and their formation is governed by pH. ATPIF1, because of its effects on ATP and heme, is important in cellular development, heme biosynthesis, energy metabolism and angiogenesis and erythrocytes differentiation. |
| Immunogen【免疫原】 | Epitope: N-terminal inhibitory region & Antiparallel alpha-helical coiled region GST-tagged recombinant protein corresponding to the N-terminal inhibitory region & Antiparallel alpha-helical coiled eegion of human ATPIF1. |
| Application【应用】 | Research Sub Category Apoptosis - Additional This Anti-ATPIF1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of ATPIF1. Research Category Apoptosis & Cancer Western Blotting Analysis: 2.0 µg/mL from a representative lot detected ATPIF1 in 10 µg of HEK293 cell lysate. Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected ATPIF1 in HeLa, A431, and NIH/3T3 cells. |
| Quality【质量】 | Evaluated by Western Blotting in T47D cell lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected ATPIF1 in 10 µg of T47D cell lysate. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | mouse, human |
| technique(s) | immunocytochemistry: suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_057395 |
| UniProt accession no.【UniProt登记号】 | Q9UII2 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... ATPIF1(93974) |
产品说明
| Target description【目标描述】 | ~12 kDa observed. Uncharacterized band(s) may appear in some lysates. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
