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Anti-Astrocytomas Antibody, a marker for Low Grade Astrocytoma, clone J1-31
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
Specificity【特异性】 | The antibody recognizes an intracellular protein antigen (MW 30 kDa) expressed by human and rat astrocytes and other specialized glia (Muller cells of the retina, Bergmann fibers of the cerebellar cortex, tanycytes of the hypothalamus and ciliated ependymal cells) in the central nervous system (CNS).The antibody has recently been found to be a specific marker for low grade astrocytoma in human brain tissue. The antibody is able to distinguish between low grade astrocytoma and normal reactive gliosis (patent application filed). Monoclonal antibody J1-31 was raised against crude homogenate of brain tissue from a multiple sclerosis (MS) patient (autopsy sample; Malhotra et al.: Microbios Letters 26:151-157, 1984). In human brain, MAb J1-31 recognizes an intracellular protein antigen (J1-31 antigen), which bands at approximately 30,000 daltons under reducing conditions for sodium dodecyl sulfate gel electrophoresis (Singh et al.: Bioscience Reports 6:73-79, 1986). By immunofluorescence microscopy, MAb J1-31 stains those cells that are also stained by antiserum to glial fibrillary acidic protein (GFAP), namely astrocytes, retinal Muller cells, and tanycytes in the ependyma (Predy et al.: Bioscience Reports 7:491-502, 1987). In addition, MAb J1-31 stains ciliated ependymal cells that do not express GFAP {Malhotra, SK (1989) J Neurosci Res. 22(1):36-49}. |
Immunogen【免疫原】 | Human cerebral white matter plaque materials from a multiple sclerosis patient. Epitope: a marker for Low Grade Astrocytoma |
Application【应用】 | Research Sub Category Neuronal & Glial Markers Detect Astrocytomas using this Anti-Astrocytomas Antibody, a marker for Low Grade Astrocytoma, clone J1-31 validated for use in WB, IC, IH(P). Research Category Neuroscience Western blot: reducing conditions, recognizes a 30kDa protein {Singh, R et al (1986) Biosci Rep 6(1):73-80. Immunohistochemistry The antibody works on paraffin embedded tissue sections. Immunocytochemistry Optimal working dilutions must be determined by the end user. |
Linkage【联系】 | Replaces: MAB5314 |
Physical form【外形】 | Ascites fluid. Liquid. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | ascites fluid |
antibody product type | primary antibodies |
clone【克隆】 | J1-31, monoclonal |
species reactivity | mouse, rat, guinea pig, human |
manufacturer/tradename | Chemicon® |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable (paraffin) western blot: suitable |
isotype【同位素/亚型】 | IgM |
shipped in【运输】 | dry ice |
产品说明
Storage and Stability【储存及稳定性】 | Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |