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Anti-Astrin/SPAG5, clone 8B2 Antibody
产品别名
Anti-Astrin/SPAG5, clone 8B2 Antibody
Sperm-associated antigen 5, Astrin, Deepest, Mitotic spindle-associated protein p126, MAP126
基本信息
eCl@ss | 32160702 |
General description【一般描述】 | Astrin/SPAG5 is a microtubule binding and cytoskeleton associated signaling protein that plays not only a critical role in mitotic spindle and chromosome segregation but also as an important signal transducer in mTOR and GSK3 beta signaling pathways. Astrin/SPAG5 is localized in the cytoplasm and/or mitotic apparatus (in dividing cells) and centrosomes. In non-dividing cells Astrin/SPAG5 is associated with microtubules unless under cellular stress, then it is localized in stress granules. Astrin/SPAG5 is expressed in many tissues but is very high in testis and sperm and is regulated via the cell cycle highest levels are seen in prophase. Recent research has focused on Astrin/SPAG5 critical role in regulating mTORC1 and its effects on growth and survival. When cells are stress Astrin/SPAG5 inhibits mTORC1 association and recruits the mTORC1 component raptor to stress granules (SGs), thereby preventing mTORC1-hyperactivation-induced apoptosis. Because cancerous tumor cells are often stressed, this mechanism is believed to be key to their survival, thus Astrin/SPAG5 activity repression is being examined as potential anti-cancer therapy. |
Immunogen【免疫原】 | Linear peptide corresponding to human Astrin/SPAG5. |
Application【应用】 | Research Sub Category Apoptosis - Additional Immunocytochemistry Analysis: A representative lot detected Astrin/SPAG5 HeLa cells (Thedieck, K., et al. (2013). Cell. 154:859-874). Western Blotting Analysis: A representative lot detected Astrin/SPAG5 in SiAstrin (Dr. Elisabeth Kremmer). Immunoprecipitation Analysis: A representative lot immunoprecipitated Astrin/SPAG5 in SiAstrin (Dr. Elisabeth Kremmer). This Anti-Astrin/SPAG5, clone 8B2 Antibody is validated for use in Immunocytochemistry and Western Blotting and Immunoprecipitation for the detection of Astrin/SPAG5. Research Category Apoptosis & Cancer |
Quality【质量】 | Evaluated by Immunocytochemistry in HeLa and A431 cells. Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected Astrin/SPAG5 in HeLa and A431 cells. |
Physical form【外形】 | Format: Purified Purified rat monoclonal IgG2cκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Protein G Purified |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | rat |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | 8B2, monoclonal |
species reactivity | human |
technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG2cκ |
NCBI accession no.【NCBI登记号】 | NP_006452 |
UniProt accession no.【UniProt登记号】 | Q96R06 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | 134 kDa calculated |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |