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Anti-Aiolos Antibody, clone 9D10
产品别名
Anti-Aiolos Antibody, clone 9D10
Zinc finger protein Aiolos, Ikaros family zinc finger protein 3
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Aiolos is a zinc finger DNA binding transcription factor important in lymphocyte differentiation and development. Aiolos plays an essential role in the regulation of B-cell differentiation including, proliferation and maturation to plasma cells. Aiolos also works as a cofactor in gene suppression via its interaction with the transcription factor FOXP3 in regulatory T cells as well, and Aiolos is involved in various chromatin functions too through its interaction with histone deacetylase complexes containing HDAC1, MTA2 and SIN3A. Aiolos is localized in the nucleus primarily but can be found in the cytoplasm depending upon the cellular situation. Aiolos is expressed in leukocytes and found in high concentration in spleen and thymus and has multiple isoforms. Activity is regulated via phosphorylation. |
Immunogen【免疫原】 | Recombinant protein corresponding to human Aiolos. |
Application【应用】 | Research Sub Category Transcription Factors Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to work in ChIP (see Zhang, J., et al 2012). ChIP-seq: A representative lot of this antibody was reported to work in ChIP (see Zhang, J., et al 2012). Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Aliolos in an HL-60 cell lysate This Anti-Aiolos Antibody, clone 9D10 is validated for use in Western Blotting and ChIP-seq and Chromatin Immunoprecipitation (ChIP) for the detection of Aiolos. Research Category Epigenetics & Nuclear Function |
Quality【质量】 | Evaluated by Western Blotting in a Jurkat cell lysate. Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Aliolos in 10 µg of a Jurkat cell lysate. |
Physical form【外形】 | Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified Protein G Purified |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | 9D10, monoclonal |
species reactivity | human, mouse |
technique(s) | ChIP: suitable (ChIP-seq) western blot: suitable |
isotype【同位素/亚型】 | IgG2bκ |
NCBI accession no.【NCBI登记号】 | NP_001244337 |
UniProt accession no.【UniProt登记号】 | Q9UKT9 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | ~ 58-70 kDa observed |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |