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Anti-Acylation Stimulating Protein Antibody, clone 4H3
产品别名
Anti-Acylation Stimulating Protein Antibody, clone 4H3
Complement C3, C3adesArg, C3, PZP-like alpha-2-macroglobulin domain-containing protein 1
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | The protein called Acylation stimulating protein (ASP) is a cleavage fragment protein from the Complement C3 gene. C3 plays a central role in the activation of the complement system. C3’s processing by C3 convertase is the central reaction in both classical and alternative complement pathways. ASP acts as an adipogenic hormone that stimulates triglyceride synthesis and glucose transport in adipocytes, and thus it helps regulate fat storage and triglyceride synthesis. ASP stimulates triglyceride synthesis via PLC, MAPK and AKT signaling pathways and ASP promotes the phosphorylation and internalization and recycling of Complement factor C5AR2. ASP is expressed in adipocytes and released into the plasma during both the fasting and postprandial periods. Increased levels of C3 and its cleavage product ASP are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage. |
Immunogen【免疫原】 | linear peptide corresponding to Human Acylation Stimulating Protein. |
Application【应用】 | ELISA Analysis: 7 µg/mL of this antibody detected ASP (PEG precipitated) in a sandwich ELISA format (Saleh, J,. et al, J. Lipid Res. 1998. 39: 884–891) Research Sub Category Insulin/Energy Signaling Lipid Metabolism & Weight Regulation Research Category Signaling Metabolism This Anti-Acylation Stimulating Protein Antibody, clone 4H3 is validated for use in Western Blotting and ELISA for the detection of Acylation Stimulating Protein. |
Quality【质量】 | Evaluated by Western Blotting on purified Acylation Stimulating Protein from transfected HEK293 cell lysate Western Blotting Analysis: 0.1 µg/mL of this antibody detected purified Acylation Stimulating Protein from transfected HEK293 cell. |
Physical form【外形】 | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified Protein G Purified |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | 4H3, monoclonal |
species reactivity | human |
technique(s) | ELISA: suitable western blot: suitable |
isotype【同位素/亚型】 | IgG1κ |
NCBI accession no.【NCBI登记号】 | NP_000055 |
UniProt accession no.【UniProt登记号】 | P01024 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | ~ 16/10 kDa observed, 10 kDa indicates native ASP, 16 kDa indicates HIS-Tag ASP. HIS-Tag was added for purification purposes. We have found no difference in Activity (measured by TGS, Fatty Acid uptake, etc) between rASP with/without the tag. Further, the ELISA was successful in measuring both. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |