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Anti-Acylation Stimulating Protein Antibody, clone 4H3

产品编号:4104596
规格:clone 4H3, from mouse
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4104596100 μG5850
产品别名

Anti-Acylation Stimulating Protein Antibody, clone 4H3

Complement C3, C3adesArg, C3, PZP-like alpha-2-macroglobulin domain-containing protein 1

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
The protein called Acylation stimulating protein (ASP) is a cleavage fragment protein from the Complement C3 gene. C3 plays a central role in the activation of the complement system. C3’s processing by C3 convertase is the central reaction in both classical and alternative complement pathways. ASP acts as an adipogenic hormone that stimulates triglyceride synthesis and glucose transport in adipocytes, and thus it helps regulate fat storage and triglyceride synthesis. ASP stimulates triglyceride synthesis via PLC, MAPK and AKT signaling pathways and ASP promotes the phosphorylation and internalization and recycling of Complement factor C5AR2. ASP is expressed in adipocytes and released into the plasma during both the fasting and postprandial periods. Increased levels of C3 and its cleavage product ASP are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.
Immunogen【免疫原】
linear peptide corresponding to Human Acylation Stimulating Protein.
Application【应用】
ELISA Analysis: 7 µg/mL of this antibody detected ASP (PEG precipitated) in a sandwich ELISA format (Saleh, J,. et al, J. Lipid Res. 1998. 39: 884–891)
Research Sub Category
Insulin/Energy Signaling

Lipid Metabolism & Weight Regulation
Research Category
Signaling

Metabolism
This Anti-Acylation Stimulating Protein Antibody, clone 4H3 is validated for use in Western Blotting and ELISA for the detection of Acylation Stimulating Protein.
Quality【质量】
Evaluated by Western Blotting on purified Acylation Stimulating Protein from transfected HEK293 cell lysate

Western Blotting Analysis: 0.1 µg/mL of this antibody detected purified Acylation Stimulating Protein from transfected HEK293 cell.
Physical form【外形】
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified
Other Notes【其他说明】
Concentration: Please refer to lot specific datasheet.
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
4H3, monoclonal
species reactivity
human
technique(s)
ELISA: suitable
western blot: suitable
isotype【同位素/亚型】
IgG1κ
NCBI accession no.【NCBI登记号】
NP_000055
UniProt accession no.【UniProt登记号】
P01024
shipped in【运输】
wet ice
产品说明
Target description【目标描述】
~ 16/10 kDa observed, 10 kDa indicates native ASP, 16 kDa indicates HIS-Tag ASP. HIS-Tag was added for purification purposes. We have found no difference in Activity (measured by TGS, Fatty Acid uptake, etc) between rASP with/without the tag. Further, the ELISA was successful in measuring both.
Storage and Stability【储存及稳定性】
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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