您的位置:首页 > 产品中心 > Anti-Actin Antibody, clone JLA20
Anti-Actin Antibody, clone JLA20
产品别名
Anti-Actin Antibody, clone JLA20
Actin, alpha skeletal muscle, Alpha-actin-1
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Actin is an abundantly expressed in eukaryotic cells and it is the core component of the cytoskeleton. It exists in three isoforms: alpha actin, which is found in muscle tissue and forms fibrous actin that facilitates muscle contractility; and beta and gamma actin which are found in non-muscle cells and facilitate cell motility. Actin exists initially as globular actin (G-actin) which is converted to filamentous actin (F-actin) upon assembly. The formation of F-actin is a complex multistep process that is tightly regulated by signaling from the actin-binding proteins (ABPs) and Rho family GTPases. Filamentous actin is crosslinked with other actin polymers to form a complex cytoskeletal network which is required for exo- and phagocytosis, vesicular trafficking, cell migration, and gene regulation, among other cellular processes. |
Application【应用】 | Detect Actin using this Anti-Actin Antibody, clone JLA20 validated for use in Western Blotting, Immunofluorescence. Immunofluorescence Analysis: A representative lot was used by an independent laboratory in NRK cells. (Cao, L.G., et al. (1993). The Journal of Cell Biology. 123(1):173-181.) Western Blot Analysis: A representative lot was used by an independent laboratory in Me4405 melanoma cell lysate. (Thomas, W.D., et al. (2000). Journal of Immunology. 165:5612–5620.) |
Quality【质量】 | Evaluated by Western Blot in SK-BR3 cell lysate. Western Blot Analysis: A 1:1,000 dilution of this antibody detected Actin on 10 µg of SK-BR3 cell lysate. |
Linkage【联系】 | Replaces: 04-1040 |
Physical form【外形】 | Purified by Ion-Exchange Chromatography Format: Purified |
Analysis Note【分析说明】 | Control SK-BR3 cell lysate |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | JLA20, monoclonal |
species reactivity | human |
species reactivity (predicted by homology) | chicken (immunogen homology) |
technique(s) | immunofluorescence: suitable western blot: suitable |
isotype【同位素/亚型】 | IgMκ |
NCBI accession no.【NCBI登记号】 | NP_001026234 |
UniProt accession no.【UniProt登记号】 | P68139 |
shipped in【运输】 | wet ice |
Gene Information | human ... ACTA1(58) |
产品说明
Target description【目标描述】 | ~42 kDa observed |
【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 2 |