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ANG-2 Protein, Human Recombinant
产品编号: | 4104143 |
规格: | Angiopoietin2 (Ang-2) is a secreted glycoprotein that plays a complex role in angiogenesis & inflammation. |
包装规格: | 20 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
ANG-2 Protein, Human Recombinant
ANG2, ANG, Angiopoietin 2, ANGPT2
基本信息
eCl@ss | 32160405 |
General description【一般描述】 | Product Source: CHO Cells Angiopoietin2 (Ang-2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies. ANG-2 binds to the endothelial cell specific receptor Tie2, but, in contrast to ANG-1 does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie2 and, depending on the physiological and biochemical environment, can act either as a n agonist or antagonist of Tie2 induced angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an α helix rich “coiled coil” N-terminal domain and fibrinogen like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer. Recombinant human ANG-2 is a C-terminal histidine tagged glycoprotein which migrates with an apparent molecular mass of 60.0 – 70.0 kDa by SDS-PAGE under reducing conditions. Sequencing analysis shows an N-terminal sequence starting with residue 68 (D) of the ANG-2 precursor protein. |
Specificity【特异性】 | Cross Reactivty None |
Application【应用】 | Research Category Stem Cell Research Research Sub Category Growth Factors & Receptors |
Quality【质量】 | Bioactivity assay: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC). Bioactivity assay: Determined by its ability to stimulate tubulogenesis in HUVEC cells using a concentration of 0.2μg/ml. |
Physical form【外形】 | Product is filtered through a 0.2 micron filter before lyophilization. |
产品性质
Quality Level【质量水平】 | 100 |
Assay【测定】 | >95% (SDS-PAGE and HPLC) |
impurities【杂质】 | <0.1 μg/mg protein endotoxin (<1EU/μg) |
UniProt accession no.【UniProt登记号】 | O15123 |
shipped in【运输】 | dry ice |
产品说明
Storage and Stability【储存及稳定性】 | Store at -20°C for up to 6 months from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 1 |