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Agrin Human
产品编号: | 4103913 |
规格: | Recombinant, cell culture tested, expressed in HEK 293 cells |
包装规格: | 50 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Agrin Human
AGRN
Proteoglycan
产品性质
recombinant【重组】 | expressed in HEK 293 cells |
Assay【测定】 | ≥95% (SDS-PAGE) |
form【形式】 | lyophilized powder |
potency【效能】 | attachment assay, measured by the ability of the immobilized agrin to support the adhesion of PC12 cells |
technique(s) | cell culture | mammalian: suitable |
suitability【适用性】 | endotoxin tested |
shipped in【运输】 | ambient |
storage temp.【储存温度】 | −20℃ |
基本信息
General description【一般描述】 | Agrin C-terminal human recombinant is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 87 kDa (amino acids Ala1260-Pro2045, with an N-terminal His tag). The DTT-reduced protein migrates as a ∼100 kDa polypeptide on SDS-PAGE due to glycosylation. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher bioactivity of the protein. |
Biochem/physiol Actions【生化/生理作用】 | Agrin is a large extracellular heparan sulfate proteoglycan that is involved in the formation of the neuromuscular junction. Agrin triggers the aggregation of acetylcholine receptors via the muscle-specific kinase (MuSK) and the low-density lipoprotein receptor-related protein 4 (Lrp4) receptor complex. Agrin is required for the full regenerative capacity of neonatal mouse hearts. Recombinant agrin has been shown in vitro to promote cardiomyocyte proliferation, as studied in mouse-induced and human-induced pluripotent stem cells. Recombinant agrin has been shown in vivo to promote cardiac regeneration, after induction of myocardial infarction in adult mice. |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 2 |