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Acetylcholine Assay Kit

产品编号:4103857
规格:Sufficient for 100 colorimetric or fluorometric tests
包装规格:1 kit
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
41038571 kit5320
产品性质
Quality Level【质量水平】
100
application(s)
pharmaceutical
Detection Method
colorimetric
fluorometric
relevant disease(s)【相关疾病】
cardiovascular diseases, Alzheimer′s disease; neurological disorders; neurodegenerative disorders
storage temp.【储存温度】
−20℃
suitability【适用性】
The kit is suitable for the quantitative determination of acetylcholine and evaluation of drug effects on acetylcholine metabolism in serum, plasma, urine, saliva, milk, tissue, and cell culture etc.
基本信息
General description【一般描述】
Acetylcholine is a neurotransmitter produced in acetylcholinergic neurons. It plays important roles in skeletal muscle movement, regulation of smooth and cardiac muscles, as well as in learning, memory and mood.
Features and Benefits【特点和优势】
  • 20 μL sample size.
  • Linear detection range: colorimetric assay 10 to 200 μM, fluorimetric assay 0.4 to 10 μM acetylcholine.
Principle【原理】
The Acetylcholine Assay Kit provides a simple, direct and high-throughput assay for measuring acetylcholine in biological samples. In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase tobetaine and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The color intensity at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the acetylcholine concentration in the sample.
安全信息
Hazard Statements
H412
Precautionary Statements
P273 - P501
Hazard Classifications【危险分类】
Aquatic Chronic 3
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)【闪点(F)】
188.6 °F
Flash Point(C)【闪点(C)】
87 ℃
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