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Acetaldehyde Assay Kit

产品编号:4103839
规格:Sufficient for 100 colorimetric tests
包装规格:1 kit
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
41038391 kit3980
产品性质
Quality Level【质量水平】
100
application(s)
agriculture
food and beverages
Detection Method
colorimetric
storage temp.【储存温度】
−20℃
suitability【适用性】
The kit is suitable for acetaldehyde determination in biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice).
基本信息
General description【一般描述】
Acetaldehyde (CH3CHO) is one of the most widely occurring aldehydes in nature and is commonly used in industry. Acetaldehyde, a metabolic byproduct of ethanol in the liver, is toxic to the human body and is rapidly converted to the less harmful acetic acid by the enzyme aldehyde dehydrogenase. People with a deficiency of aldehyde dehydrogenase accumulate acetaldehyde when consuming alcohol and this accumulation results in facial and body flushing often referred to as “Asian flush syndrome.” Buildup of acetaldehyde has also been associated with the effects of hangovers from alcohol consumption. Although classified as a carcinogen, acetaldehyde is naturally found in many foods and beverages such as ripe fruit, coffee, and wine.
Features and Benefits【特点和优势】
  • Linear detection range (20 μL sample): 2 μM to 2mM acetaldehyde in 96-well plate assay.
  • Convenient room temperature assay. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes.
  • Compatible with high-throughput handling systems.
Principle【原理】
The Acetaldehyde Assay Kit is based on the aldehyde dehydrogenase catalyzed oxidation of acetaldehyde with the reduction of NAD to NADH. The formed NADH subsequently reduces MTT, producing a colored formazan compound. The intensity of the end product color, measured at 565 nm, is directly proportional to the acetaldehyde concentration in the sample.
安全信息
Pictograms【象形图】
GHS07
Signal word【警示用语:】
Warning
Hazard Statements
H315 - H319
Precautionary Statements
P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
Hazard Classifications【危险分类】
Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 3
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