您的位置：首页 > 产品中心 > A2EN Human Endocervical Epithelial Cell Line
A2EN Human Endocervical Epithelial Cell Line
| 产品编号： | 4103810 |
| 规格： | A2EN endocervical epithelial cell line can be used to study sexually transmitted pathogen infections, pathogenesis and the innate immune response. |
| 包装规格： | 1X106 CELLS/VIAL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
A2EN Human Endocervical Epithelial Cell Line
A2EN Cell Line
基本信息
| eCl@ss | 32011203 |
| General description【一般描述】 | The endocervical epithelium is a single cell layer lining the endocervical canal that separates the lower female reproductive tract from the uterus and Fallopian tubes. Endocervical epithelial cells secrete mucus and innate immune mediators that facilitate reproduction as well as protect from pathogen invasion. The endocervix is a major site of transmission for multiple sexually transmitted organisms, such as Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium and human immunodeficiency virus (HIV). A2EN is an immortalized human epithelial cell line derived from human primary epithelial cells expanded from an explant of human endocervical tissue. The primary endocervical epithelial cells were immortalized with lentiviral vectors carrying the HPV E6/E7 genes. A2EN cells can generate a transepithelial electrical resistance (TEER) when grown on cell culture inserts and generate differential apical and basolateral cytokine profiles upon stimulation with various toll-like receptor (TLR) agonists. |
| Cell Line Description【细胞系描述】 | Epithelial Cells |
| Application【应用】 | A2EN endocervical epithelial cell line can be used to study sexually transmitted pathogen infections, pathogenesis and the innate immune response. This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com. Research Category Infectious Diseases Immune Response |
| Quality【质量】 | • Each vial contains ≥ 1X106 viable cells. • Cells are tested negative for Epstein-Barr virus, HPV-16, HPV-18, Hepatitis A, C, Herpesvirus type 6, 7, 8 and HIV-1 & 2 viruses by PCR • Cells are negative for mycoplasma contamination. • Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | human |
| technique(s) | cell culture | mammalian: suitable |
产品说明
| Storage and Stability【储存及稳定性】 | Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality. |
| Disclaimer【免责声明】 | This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
