您的位置:首页 > 产品中心 > 4EBP1 Active human
4EBP1 Active human
产品编号: | 4103705 |
规格: | recombinant, expressed in E. coli, ≥70% (SDS-PAGE) |
包装规格: | 100 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
4EBP1 Active human
Eukaryotic translation initiation factor 4E binding protein 1
EIF4EBP1
eIF4E-binding protein 1
PHAS-I
Phosphorylated heat- and acid-stable protein regulated by insulin 1
基本信息
MDL number | MFCD01094895 |
NACRES | NA.32 |
General description【一般描述】 | The gene E1F4EBP1 (eukaryotic translation initiation factor 4E binding protein 1) is mapped to human chromosome 8p11-p12. It belongs to the translation repressor protein family. Human E1F4EBP1 (eukaryotic translation initiation factor 4E binding protein 1)(GenBank Accession No. NM_004095), full length with N-terminal His tag, MW = 13.6kDa, expressed in Escherichia coli. |
Application【应用】 | 4EBP1 Active human has been used as a substrate for in vitro kinase assay. |
Biochem/physiol Actions【生化/生理作用】 | E1F4EBP1 (eukaryotic translation initiation factor 4E binding protein 1) is involved in protein synthesis, cell growth and survival. In unphosphorylated form, it suppresses the translation initiation of capped mRNAs. On the other hand, in phosphorylated form, it increases translation initiation. Phosphorylation of E1F4EBP1 releases it from eIF4E (eukaryotic translation initiation factor 4E), thereby resulting in cap-dependent translation. E1F4EBP1 is also involved in tumorigenesis and the gene is overexpressed in various carcinomas. It is also a substrate for mTORC1 (mammalian target of rapamycin complex 1). In non-phosphorylated conditions, it interacts with p21 (cyclin-dependent kinase inhibitor) and enhances destabilization of p21. |
Physical form【外形】 | Formulated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF and 50% glycerol. |
Preparation Note【制备说明】 | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles. |
产品性质
biological source【生物来源】 | human |
recombinant【重组】 | expressed in E. coli |
Assay【测定】 | ≥70% (SDS-PAGE) |
form【形式】 | aqueous solution |
mol wt【分子量】 | 13.6 kDa |
packaging【包装】 | pkg of 100 μg |
storage condition【储存条件】 | avoid repeated freeze/thaw cycles |
concentration【浓度】 | >0.02 mg/mL |
NCBI accession no.【NCBI登记号】 | NM_004095 |
UniProt accession no.【UniProt登记号】 | Q13541 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −70℃ |
Gene Information | human ... EIF4EBP1(1978) |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |