Anti-Tubulin Antibody, beta, clone KMX-1

32160702

NA.41

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low and therefore beta Tubulin should not be used as loading control for these tissues.

This antibody reacts specifically with β-tubulin and shows a strong cross-reactivity to different species. This antibody allows staining of microtubuli using indirect immunofluorescence showing a low background only.

Tubulin purified from polycephalum myxamoebae

Research Category
Cell Structure
This Anti-Tubulin Antibody, beta, clone KMX-1 is validated for use in IC, WB for the detection of Tubulin.
Immunocytochemistry:
A 1-2 μg/mL concentration of a previous lot was used in immunocytochemistry.

Western blot:
0.05-0.5 μg/mL

Optimal working dilutions must be determined by end user.
Research Sub Category
Cytoskeleton

Routinely evaluated by Western Blot on A431 lysates.

Western blot:
1:500 dilution of this lot detected TUBULIN (BETA) on 10 μg of A431 lysates.

Replaces: 04-1049

Format: Purified
Protein A purified
Purified mouse monoclonal IgG2b liquid in buffer containing 0.02M phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Control
HeLa Cell lysate

A431 Cell lysate

MCF7 Cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified immunoglobulin

primary antibodies

KMX-1, monoclonal

human, rat, mouse

all

Chemicon^®

immunocytochemistry: suitable
western blot: suitable

IgG2b

NM_006086.2

Q13509

wet ice

50 kDa

Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2