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Anti-Rho (-A, -B, -C) Antibody
| 产品编号： | 4102633 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-Rho (-A, -B, -C) Antibody
Transforming protein RhoA, h12, Rho-related GTP-binding protein RhoB, h6, Rho-related GTP-binding protein RhoC, h9
基本信息
| eCl@ss | 32160702 |
| General description【一般描述】 | Rac1, RhoA, and Cdc42 (UniProt P63000, P61586, and P60953, respectively) are among the most studied Rho family of small GTPases that are involved in a wide variety of cellular functions such as cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family contains 22 members which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamlly (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2). |
| Specificity【特异性】 | This polyclonal antibody is raised against an internal sequence highly conserved among RhoA (100%), RhoB (95%), and RhoC (100%). |
| Immunogen【免疫原】 | KLH-conjugated synthetic peptide (SYPDTDVILMCFSIDSPDSLEN-KK) corresponding to amino acids 73-94 of human RhoA & RhoB, with two C-terminal lysines added to increase peptide solubility corresponding to human Rho (-A, -B, -C). Epitope: Amino acids 73-94. |
| Application【应用】 | Anti-Rho (-A, -B, -C) Antibody is an antibody against Rho (-A, -B, -C) for use in Western Blotting. Research Category Signaling Research Sub Category G-proteins |
| Quality【质量】 | Evaluated by Western Blotting in HL-60 cell lysate. Western Blotting Analysis: A 1:5,000 dilution of this antibody detected RhoA family GTPase in 10 µg of HL-60 cell lysate. |
| Physical form【外形】 | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology), nematode (based on 100% sequence homology), zebrafish (based on 100% sequence homology), Xenopus (based on 100% sequence homology), mouse (based on 100% sequence homology), chicken (based on 100% sequence homology) |
| technique(s) | western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_001655 NP_004031 NP_786886 |
| UniProt accession no.【UniProt登记号】 | P08134 P61586 P62745 |
| Gene Information | human ... RHOA(387), RHOB(388) |
产品说明
| Target description【目标描述】 | ~22 kDa observed. 21.44 kDa (human RhoA), 21.78 kDa (human RhoB), and 21.68 kDa (human RhoC) calculated. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
