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产品别名
MBP, recombinant
Myelin basic protein
基本信息
eCl@ss | 32160405 |
NACRES | NA.41 |
General description【一般描述】 | Product Source: Recombinant fusion protein containing bovine myelin basic protein (MBP) with an N-terminal GST tag and a C-terminal 6His tag, expressed in E. coli. |
Quality【质量】 | Routinely evaluated by kinase assay |
Physical form【外形】 | 500 µg recombinant MBP in 500 µl of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 500 mM imidazole, 1 mM PMSF, and 10% glycerol. Frozen solution. ProBond Resin chromatography |
Analysis Note【分析说明】 | Specific Activity: This lot was successfully phosphorylated in a non-radioactive kinase assay using recombinant active MAP Kinase 2/Erk2 (Catalog # 14-173). MBP phosphorylation was detected by immunoblot analysis using anti-phospho-MBP (Catalog # 05-429). Immunoblot shown to the right. |
Other Notes【其他说明】 | Purity information is lot specific, please consult the certificate of analysis |
Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | bovine |
mol wt【分子量】 | Mw ~47 kDa |
manufacturer/tradename | Upstate® |
concentration【浓度】 | 1 mg/mL |
technique(s) | activity assay: suitable |
NCBI accession no.【NCBI登记号】 | NM_001025081.1 NM_001025090.1 NM_001025092.1 NM_001025094.1 NM_001025098.1 NM_001025100.1 NM_001025101.1 NM_002385.2 |
UniProt accession no.【UniProt登记号】 | P02686 |
shipped in【运输】 | dry ice |
产品说明
Storage and Stability【储存及稳定性】 | Stable for 2 years at -20C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |