ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye

200

ColorWheel^®

lyophilized

flow cytometry: suitable

λ_ex 651 nm; λ_em 660 nm

ambient

2-8℃

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

Allophycocyanin (APC) belongs to a family of phycobiliproteins and is well studied as a fluorescent reagent for flow cytometric analysis. It is isolated from a Spirulina species, a type of blue-green alga and is composed of α and β subunits. The heterodimers of these subunits can assemble as trimer or hexamer. APC displays high absorptivity and quantum efficiency. It has an excitation max at 651 nm and a emission max at 660 nm with extinction coefficient of 700,000 cm^-1 M^-1. It can absorb and emit light with low energy losses to quenching. APC can be easily conjugated to monoclonal and polyclonal antibodies for use in fluorescence-based detection methods, such as flow cytometry and FACS analysis. (Ref.: Sonani, RR., et al. (2015). PLoS One. 10(4): e0124580; Jung, TM., and Daily MO. (1989). J. Immunol. Methods. 121(1); 9-18).

Flow Cytometry: Excitation: 651 nm, Emission: 660 nm

CWDS-APC-25UL Set Components:

CWD-APC-25UL: ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

CWD-FINISH-25UL: ColorWheel^® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.

Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.