Anti-CREB Antibody

32160702

NA.41

CREB is a beta ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2.

Human cyclic AMP responsive element binding protein-1 (CREB-1, p43). By Western blot it shows a major band at 46 kDa when assayed against a lysate of 1 x 106 Raji B cells. Minor bands at 75 kDa and 43 kDa may be noted. Partial reactivity to CREM-1 protein. May react non-specifically with other proteins.

CREB-1 (p43) peptide corresponding to the N-terminal (aa 5-45) of human protein conjugated to KLH.

Use Anti-CREB Antibody (Rabbit Polyclonal Antibody) validated in ELISA, EMSA, IP, WB to detect CREB also known as CREB-1, cAMP-response Element-binding Protein-1.
Immunoblot

Immunoprecipitation

ELISA

Supershift assay: 0.5-1.0 μL per assay.

Optimal dilutions must be determined by the end user.

Replaces: 04-218

Purified immunoglobulin. Liquid (sterile filtered) in 0.02M Potassium Phosphate buffer containing 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide.
Format: Purified

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

purified immunoglobulin

primary antibodies

polyclonal

human, mouse, rat

Chemicon^®

ELISA: suitable
electrophoretic mobility shift assay: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NM_004379.2
NM_134442.2

P16220

wet ice

human ... CREB1(1385)

Maintain frozen at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2