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Anti-Neurofilament H Antibody, clone NE14, Alexa Fluor® 488 Conjugated
| 产品编号： | 4100018 |
| 规格： | clone NE14, from mouse, ALEXA FLUOR® 488 |
| 包装规格： | 40 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-Neurofilament H Antibody, clone NE14, Alexa Fluor® 488 Conjugated
200 kDa neurofilament protein, Neurofilament triplet H protein, neurofilament, heavy polypeptide, neurofilament, heavy polypeptide 200kDa
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure; however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. |
| Specificity【特异性】 | Reactivity with other species has not been tested. This antibody recognizes Neurofilament H. |
| Immunogen【免疫原】 | Purified neurofilament polypeptides (Debus et al., 1983). Epitope: Unknown |
| Application【应用】 | Immunohistochemistry: 5-10 µg/mL on frozen brain tissue. Optimal working dilutions must be determined by end user. Anti-Neurofilament H Antibody, clone NE14, Alexa Fluor 488 Conjugated is an antibody against Neurofilament H for use in IH & IC. Research Sub Category Neurofilament & Neuron Metabolism Research Category Neuroscience |
| Quality【质量】 | Immunocytochemical Analysis: 1:250 dilution of this lot detected Neurofilament-H in rat cortex cell culture. |
| Physical form【外形】 | Purified immunoglobulin in 0.02M Phosphate buffer, 0.25M NaCl containing 0.1% sodium azide. Protein G Purified |
| Analysis Note【分析说明】 | Control Rat Cortex Cells. |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Legal Information【法律信息】 | ALEXA FLUOR is a registered trademark of Life Technologies |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | ALEXA FLUOR® 488 |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | NE14, monoclonal |
| species reactivity | pig, human, rat, porcine |
| technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable |
| isotype【同位素/亚型】 | IgG1 |
| NCBI accession no.【NCBI登记号】 | NP_066554.2 |
| UniProt accession no.【UniProt登记号】 | P12036 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~ 200 kDa |
| Storage and Stability【储存及稳定性】 | Stable for 6 months at 2-8ºC from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
