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Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor®488 Conjugated

产品编号:4100012
规格:clone TU-20, from mouse, ALEXA FLUOR® 488
包装规格:40 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
410001240 μL7190
产品别名

Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor®488 Conjugated

Tubulin beta-4, Tubulin beta-III, tubulin, beta 3, tubulin, beta 4

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Tubulin is one of several members of a small family of globular proteins which polymerize to form cytoskeletal structures called microtubules. The most common members of the tubulin family are α-tubulin and β-tubulin. Tubulin dimers, typically composed of α- and β-tubulin subunits, assemble onto microtubules forming large polymers. β-III tubulin is a unique microtubule subunit that is found expressed almost exclusively in neurons. Due to the unique expression pattern of β-III tubulin it is often utilized as a marker to identify neuronal cells from other brain cells such as glia which do not express it. β-III tubulin also serves as a cytoarchitectural marker since it is primarily found within neurite extensions.
Specificity【特异性】
Reactivity with other species has not been determined.
This antibody reacts with the C-terminus of the beta-III isoform of tubulin, which is known to be neuron specific. Does not identify beta tubulin found in glial cells.
Immunogen【免疫原】
Epitope: C-terminus
Application【应用】
Detect Tubulin β-III using this Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor®488 Conjugated validated for use in IC, IF, WB, IH & IP.
Immunohistochemistry: 5-10 µg/mL on frozen brain tissue.
Although this conjugated antibody will work for Western blotting and Immunoprecipitation, we recommend catalog number CBL412 if these are your primary applications.
Optimal working concentration must be determined by end user.
Research Sub Category
Neurofilament & Neuron Metabolism
Research Category
Neuroscience
Quality【质量】
Routinely tested cortical neurons in Immunocytochemistry.
Immunocytochemical Analysis: 1:250 dilution of this lot detected β-III tubulin in rat cortex cell culture.
Physical form【外形】
Purified immunoglobulin in 0.02M Phosphate buffer, 0.25M NaCl containing 0.05% sodium azide.
Protein G Purified
Analysis Note【分析说明】
Control
Rat Cortex Cells.
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information【法律信息】
ALEXA FLUOR is a registered trademark of Life Technologies
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
conjugate【偶联物】
ALEXA FLUOR® 488
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
TU-20, monoclonal
species reactivity
bovine, primate, mouse, pig, human, rat
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype【同位素/亚型】
IgG1
NCBI accession no.【NCBI登记号】
NP_001184110
UniProt accession no.【UniProt登记号】
Q13509
shipped in【运输】
wet ice
产品说明
Target description【目标描述】
50 kDa
Storage and Stability【储存及稳定性】
Stable for 6 months at 2-8°C from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 2
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