Ascentis^® Express F5, 5 μm HPLC Column

32110501

SB.52

Ascentis^® Express 5 μm F5 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core^® particle design. The bonded, endcapped, dimethylpentafluorophenyl-propylsilane stationary phase of Ascentis Express 5 μm F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming π-π and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis Express 5 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

HPLC Analysis of Benzodiazepines on Ascentis^® Express F5, 5 μm

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Ascentis is a registered trademark of sigma-aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

stainless steel column

100

suitable for USP L43

Ascentis^®

endcapped

Ascentis^®

1 ea of

60 ℃ temp. range
600 bar max. pressure (9000 psi)

HPLC: suitable
LC/MS: suitable

25 cm × 2.1 mm

<5 ppm metals

Fused-Core particle platform
superficially porous particle

PFP (pentafluorophenyl) phase

5 μm

90 Å pore size

90 m²/g

2-8

food and beverages

reversed phase

13 - Non Combustible Solids

WGK 3