TrkB/Fc Chimera human

MFCD03458837

NA.32

TrkB/Fc Chimera human can be used in synaptoneurosome preparation and western blot analysis. Intracerebroventricular administeration of TrkB/Fc chimera human blocks TrkB ligand signaling and was used to study the role of BDNF in ATN response augmentation.

Member of the Trk family tyrosine kinase receptors. Binds specifically to mediate neurotrophins BDNF and NT-4/5.
TrkB/Fc Chimera human consist of extracellular domain of human TrkB1 fused to histidine-tagged Fc region of human IgG1 by using a polypeptide linker. TrkB belongs to tyrosine kinase receptors family and facilitates the hippocampal development as well as regulating the synaptic plasticity that underlies long-term potentiation and learning. Brain derived neurotrophic factor (BDNF) and Neurotrophin 4 or 5 are preferred ligands for TrkB. Nevertheless it can also bind to NTF3/neurotrophin-3 that is less efficient in activating the receptor but regulates neuron survival through NTRK2.

Extracellular domain of human TrkB fused to the C-terminal histidine-tagged Fc region of human IgG1.

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline.

Reconstitute to 100 μg/mL in phosphate buffered saline.

Inhibits BDNF-induced proliferation of TrkB transfected cells, BaF-TrkB-BD.

200

human

expressed in NSO cells

>90% (SDS-PAGE)

lyophilized powder

0.1-0.4 μg/mL ED₅₀

120-130 kDa by SDS-PAGE (reducing)
calculated mol wt 71 kDa

pkg of 100 μg

avoid repeated freeze/thaw cycles

western blot: suitable

endotoxin, tested

Q16620

−20℃

human ... NTRK2(4915)

13 - Non Combustible Solids

WGK 3