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Stromal Cell-Derived Factor 1β/pre-B Cell Growth Stimulating Factor human
| 产品编号： | 4096465 |
| 规格： | >97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture |
| 包装规格： | 10 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Stromal Cell-Derived Factor 1β/pre-B Cell Growth Stimulating Factor human
SDF-1β/PBSF
基本信息
| MDL number | MFCD01094859 |
| NACRES | NA.32 |
| General description【一般描述】 | C-X-C motif chemokine ligand 12 (CXCL12), also known as stromal cell-derived factor 1 α (SDF-1a), is encoded by the gene mapped to human chromosome 10q11. Stromal cell-derived factor 1 α and β (SDF-1 α and β) are stromal derived CXC chemokines and signal through the C-X-C motif chemokine receptor-4 (CXCR4). CXCL12 is mainly expressed in the inflamed and failing myocardium. |
| Application【应用】 | Stromal Cell-Derived Factor 1β has been used to activate chemokine receptor-4 (CXCR4) and to further assess its role in tumor cell migration using chemotaxis assay. |
| Biochem/physiol Actions【生化/生理作用】 | SDF-1/PBSF is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. It is highly conserved between species, with only one amino acid substitution between the human and mouse proteins. C-X-C motif chemokine ligand 12 (CXCL12) is involved in inflammation, proliferation, tumorigenicity and metastasis. CXCL12 exerts HIV (human immunodeficiency virus) suppressive activity in cells expressing the CXCR4 (C-X-C motif chemokine receptor 4). It is overexpressed in breast carcinoma, ovarian carcinoma and papillary thyroid carcinoma. It enhances angiogenesis in the ischemic tissue. SDF1 is also implicated in neuroprotection after ischemic stroke with the help of CXCR4. SDF-1/PBSF, a member of the α (CXC) chemokine family, is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1α and SDF-1β (together also known as SDF-1/PBSF) are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the C-terminal of SDF-1β and absent from SDF-1α. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the human and mouse proteins. |
| Physical form【外形】 | Lyophilized from 30% acetonitrile and 0.1% trifluoracetic acid containing 0.5 mg of bovine serum albumin. |
| Analysis Note【分析说明】 | The biological activity is measured by its ability to chemoattract 8-10 day human T lymphocytes cultured in the presence of IL-2. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in E. coli |
| Assay【测定】 | >97% (SDS-PAGE) |
| form【形式】 | lyophilized powder |
| potency【效能】 | 10-30 ng/mL ED50 |
| mol wt【分子量】 | ~8 kDa |
| packaging【包装】 | pkg of 10 μg |
| storage condition【储存条件】 | avoid repeated freeze/thaw cycles |
| technique(s) | cell culture | mammalian: suitable |
| impurities【杂质】 | endotoxin, tested |
| UniProt accession no.【UniProt登记号】 | P48061 |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... CXCL12(6387) |
安全信息
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 3 |
